山西地区鸽圆环病毒全基因组序列的测定与分析  

Genome sequencing and analysis of pigeon circovirus from the Shanxi Province

作　　者：张娟[1] 任鹏飞 李兴 高振 王仲兵[1] 梁立滨 ZHANG Juan;REN Peng-fei;LI Xing;GAO Zhen;WANG Zhong-bing;LIANG Li-bin(Shanxi Provincial Animal Disease Prevention and Control Center,Taiyuan 030000,China;College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西省动物疫病预防控制中心,山西太原030000 [2]山西农业大学动物医学学院,山西太谷030801

出　　处：《中国预防兽医学报》2025年第1期96-101,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：山西省基础研究计划面上项目(202103021224156);山西农业大学博士科研启动项目(2021BQ78)。

摘　　要：为了解山西地区鸽圆环病毒(PiCV)的遗传演化特征,本研究从山西太原、晋中等地区鸽养殖场采集10份疑似感染PiCV的病料样品经常规处理后进行Cap基因的PCR检测。结果显示,从10份病料样品中检测到1份PiCV阳性样品,将鉴定的PiCV命名为SX01/Shanxi/2023株。通过PCR分段扩增SX01/Shanxi/2023株的全基因组序列并测序,利用SeqMan软件将序列拼接。结果显示,分别获得759bp和1560bp的目的片段,经测序拼接后获得了PiCV全长2038bp的全基因组序列。利用MegAlign软件分析PiCV全基因组序列的同源性;采用IQ-tree软件构建PiCV全基因组的遗传进化树;利用ClusterW软件分析PiCV全基因组编码氨基酸序列的变异;利用Simplot和RDP4软件对SX01/Shanxi/2023株进行重组分析。结果显示:SX01/Shanxi/2023株与国内外PiCV全基因组序列的同源性在84.6%~96.3%,其中与德国PiCVCoCV株同源性最高达95.1%,与我国陕西TF1/SN/2016株的同源性最高达96.3%。进化树结果显示,SX01/Shanxi/2023株与国内陕西TF1/SN/2016株及DS1/GS/2018株处于同一进化分支,亲缘关系较近。氨基酸序列分析结果显示,SX01/Shanxi/2023株Cap蛋白及Rep蛋白氨基酸序列与PiCV参考株相比均发生了部分氨基酸位点的插入、缺失或突变,其中Cap蛋白氨基酸序列变异多于Rep蛋白。重组分析结果显示,该株病毒是由陕西TF1/SN/2016株为主要亲本,陕西WL1/SN/2018株为次要亲本形成的重组病毒,重组断点位于nt520。基于IQ-tree软件构建的重组断裂点位点前后(nt1~nt520、nt521~nt2038)的进化树进一步验证了上述结果。本研究首次在山西地区检测到PiCV,丰富了PiCV分子生物学特征和流行病学研究内容,也为山西地区PiCV感染的防控提供了重要参考依据。To investigate the genetic evolution characteristics of pigeon circovirus(PiCV)in Shanxi Province,this study collected 10 suspected PiCV-infected samples from pigeon farms in Taiyuan,Jinzhong,and other regions of Shanxi.PCR detection revealed that one PiCV-positive sample was detected from 10 tested samples,and named the identified PiCV as SX01/Shanxi/2023 strain.The full genome sequence of the SX01/Shanxi/2023 strain was amplified in fragments using PCR and sequenced,and the sequences were spliced using SeqMan software.Two target fragments of 759bp and 1560bp were obtained respectively.After sequencing and splicing,the complete genome sequence of PiCV was obtained,and the total genome length was 2038bp.The homology of the identified PiCV genome sequence was analyzed using MegAlign software,and a phylogenetic tree of the PiCV genome was constructed using IQ-tree software.Amino acid sequence variations in the PiCV genome were analyzed by Cluster W software.Recombination analysis of the SX01/Shanxi/2023 strain was performed using Simplot and RDP4 software.The results showed that the SX01/Shanxi/2023 strain shared 84.6%-96.3%homology with PiCV genome sequences from domestic and abroad strains.Among these,the highest homology(95.1%)was observed with the German PiCV CoCV strain,and the homology with domestic Shaanxi TF1/SN/2016 strain was as high as 96.3%.The phylogenetic analysis revealed that the SX01/Shanxi/2023 strain was clustered in the same evolutionary branch as the domestic Shaanxi TF1/SN/2016 and DS1/GS/2018 strains,indicating a close genetic relationship.The results of amino acid sequence analysis showed that the SX01/Shanxi/2023 strain exhibited insertions,deletions or mutations at some amino acid sites in both the Cap and Rep proteins compared to the PiCV reference strain,Notably,greater variation was observed in the Cap protein than in the Rep protein.Recombination analysis indicated that the SX01/Shanxi/2023 strain is a recombinant virus,with the Shaanxi TF1/SN/2016 strain as the major parent and the Sh

关 键 词：鸽圆环病毒 全基因组 遗传进化分析 重组分析 

分 类 号：S852.65[农业科学—基础兽医学]