肠道菌群与食物过敏的因果关联:一项孟德尔随机化分析  

Causal association between gut microbiota and food allergy:a Mendelian randomization analysis

作　　者：胡丽鑫 范国振 马慧[1] 李蕾[1] 王芳[1] 曲政海[1] 管仁政[1] HU Li-Xin;FAN Guo-Zhen;MA Hui;LI Lei;WANG Fang;QU Zheng-Hai;GUAN Ren-Zheng(Department of Pediatric Respiratory and Cardiovascular Medicine,Affiliated Hospital of Qingdao University,Qingdao,Shandong 266555,China)

机构地区：[1]青岛大学附属医院儿童呼吸心血管内科,山东青岛266555

出　　处：《中国当代儿科杂志》2025年第4期444-450,共7页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金项目(82300389);山东省医药卫生科技发展计划项目(202212070731)。

摘　　要：目的通过两样本孟德尔随机化(Mendelian randomization,MR)的科研方法,分析肠道菌群与食物过敏(food allergy,FA)之间的可能因果关系。方法使用肠道菌群和FA的全基因组关联分析数据库数据,通过MR分析,采用逆方差加权法、MR-Egger回归法、加权中位数法评估肠道菌群与FA的因果关系。采用Cochrane's Q检验评估工具变量异质性,MR-PRESSO分析测试异常值和多效性,MR-Egger回归评估水平多效性。使用“留一法”评估去除单个单核苷酸多态性后对因果关系的影响。结果逆方差加权法分析结果显示,疣微菌纲、疣微菌科、疣微菌目、Ruminococcaceae UCG013属和阿克曼氏菌属均与FA呈负相关(P<0.05)。敏感性分析显示结果可靠,不存在异质性和多效性。结论肠道菌群与FA存在因果关系,疣微菌纲、疣微菌科、疣微菌目、Ruminococcaceae UCG013属和阿克曼氏菌属可能降低FA的发病风险,为FA的治疗和预防提供了靶向调控目标,但仍需进一步研究来探究这些菌群影响FA的具体作用机制。Objective To analyze the potential causal relationship between gut microbiota and food allergy(FA)using two-sample Mendelian randomization(MR)methods.Methods Data from genome-wide association studies on gut microbiota and FA were utilized.MR analysis was conducted employing inverse variance weighting,MR-Egger regression,and weighted median methods to assess the causal relationship between gut microbiota and FA.Cochrane's Q test was used to evaluate heterogeneity of instrumental variables,MR-PRESSO analysis was conducted to test for outliers and pleiotropy,and MR-Egger regression was employed to assess horizontal pleiotropy.The"leave-one-out"method was used to evaluate the impact of removing individual single nucleotide polymorphisms on the causal relationship.Results Inverse variance weighting analysis revealed that the phylum Verrucomicrobia,family Verrucomicrobiaceae,order Verrucomicrobiales,genus Ruminococcaceae UCG013,and genus Akkermansia were negatively associated with FA(P<0.05).Sensitivity analyses confirmed the reliability of the findings,indicating no heterogeneity or pleiotropy present.Conclusions There is a causal relationship between gut microbiota and FA,with Verrucomicrobia,Verrucomicrobiaceae,Verrucomicrobiales,Ruminococcaceae UCG013,and Akkermansia potentially reducing the risk of developing FA.These findings provide potential targets for the treatment and prevention of FA;however,further research is needed to explore the specific mechanisms by which the microbiota influence FA.

关 键 词：食物过敏 孟德尔随机化 肠道菌群 疣微菌纲 RuminococcaceaeUCG013属 阿克曼氏菌属 

分 类 号：R446.5[医药卫生—诊断学]