等温扩增荧光法快速鉴定猪肉掺假  

Rapid Identification of Pork Adulteration by Isothermal Amplification Fluorescence Method

作　　者：姜仁普 梁振瑞 张薇 段真文 许绍坤 JIANG Renpu;LIANG Zhenrui;ZHANG Wei;DUAN Zhenwen;XU Shaokun(Yunnan Rare Biotechnology Co.,Ltd.,Kunming 650000,China)

机构地区：[1]云南瑞栢泰生物科技有限公司,云南昆明650000

出　　处：《现代食品》2025年第4期210-212,219,共4页Modern Food

基　　金：分子检测诊断产品关键技术研发(202102AA310028)。

摘　　要：本研究为建立快速鉴定猪肉掺假的等温扩增荧光法,实现对猪肉的快速检测,利用PG1引物组结合其他必需的试剂,建立一种等温扩增荧光检测方法。结果表明,本研究设计建立的针对猪肉掺假检测方法,具有特异性和灵敏度好,能够用于猪肉掺假的快速鉴别。In order to establish an isothermal amplification fluorescence method for the rapid identification of pork adulteration and realize the rapid detection of pork,an isothermal amplification fluorescence detection method was established by using PG1 primer and other necessary reagents.The results showed that the detection method for pork adulteration designed in this study has good specificity and sensitivity,and can be used for rapid identification of pork adulteration.

关 键 词：等温扩增荧光法 肉掺假 特异性 灵敏度 

分 类 号：O657.3[理学—分析化学]