2021-2023年安徽省两市发热伴血小板减少综合征病毒全基因组序列特征分析  

Genetic characteristics of whole genome sequences of severe fever with thrombocytopenia syndrome virus in two cities of Anhui,2021-2023

作　　者：陈晴晴 吴小珉 陆皖行 储娜 李嘉琪 龚磊[1] 孙永[1] Chen Qingqing;Wu Xiaomin;Lu Wanhang;Chu Na;Li Jiaqi;Gong Lei;Sun Yong(Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,Anhui,China)

机构地区：[1]安徽省疾病预防控制中心,安徽合肥230601

出　　处：《疾病监测》2025年第2期214-219,共6页Disease Surveillance

基　　金：2022年度安徽省卫生健康科研项目(No.AHWJ2022b016,No.AHWJ2022b030)。

摘　　要：目的分析安徽省滁州市和安庆市2021—2023年分离获得的发热伴血小板减少综合征病毒(SFTSV)全基因组序列特征,为发热伴血小板减少综合征(SFTS)防控提供科学依据。方法选取2021—2023年安徽省滁州、安庆市分离的SFTSV毒株共55株进行全基因组序列测序,采用MEGA 7.01、DNASTAR 7.1和RDP 4.101等软件对获取的SFTSV的L、M、S片段序列进行比对分型、相似性分析、基因重组分析和进化压力分析等。结果55株SFTSV毒株分为A、C、E和F四种基因型,3种重配型。滁州市和安庆市主要流行基因型分别为A和F型。55株SFTSV的L、M、S片段核苷酸序列相似性分别为95.40%~100.00%、93.40%~99.90%和94.20%~100.00%;RNA依赖的RNA聚合酶(RdRp)、糖蛋白前体(GP)、核蛋白(NP)和非结构蛋白(NSs)氨基酸序列相似性分别为99.20%~100.00%、97.30%~100.00%、98.80%~100.00%和96.60%~100.00%。3株SFTSV毒株的S序列存在基因重组现象。55株SFTSV毒株的RdRp、GP、NP和NSs的非同义替换率与同义替换率的比值(dN/dS)分别为0.05、0.14、0.03和0.13。结论安徽省滁州市和安庆市SFTSV毒株存在多种基因型并流行的情况,应加强SFTS病例的病原学监测工作,为安徽省SFTS防控工作提供研究基础。Objective To analyze the genetic characteristics of the whole genome sequences of severe fever with thrombocytopenia syndrome virus(SFTSV)in Chuzhou and Anqing of Anhui province from 2021 to 2023 and provide evidence for the prevention and control of severe fever withthrombocytopenia syndrome(SFTS).Methods A total of 55 SFTSV strains were collected in Chuzhou and Anqing during 2021-2023 for whole genome sequence amplification and analysis.The L,M and S segments of the strains were analyzed by software MEGA 7.01,DNASTAR 7.1 and RDP 4.101 to identify the genotype,sequence similarity,genetic recombination and selection pressure.Results Genotype A,C,E,F and 3 reassortment forms were found in 55 SFTSV strains.The major genotypes in Chuzhou and Anqing were A and F,respectively.The similarities of L,M,and S segments of the strains ranged from 95.40%to 100.00%,93.40%to 99.90%,and 94.20%to 100.00%,respectively.The viral RNA-dependent RNA polymerase(RdRp),glycoprotein precursor(GP),nucleocapsid(NP)and nonstructural protein(NSs)amino acid sequence similarities were 99.20%-100.00%,97.30%-100.00%,98.80%-100.00%,and 96.60%-100.00%,respectively.Genetic recombination occurred in the S segment of 3 SFTSV strains.The ratios of substitution rates at non-synonymous versus synonymous sites of the RdRp,GP,NP and NSs were 0.05,0.14,0.03 and 0.13,respectively.Conclusion Multiple genotypes of SFTSV were detected in Chuzhou and Anqing cities,indicating that it is necessary to strengthen the etiological surveillance for SFTS to provide basic data for the prevention and control of SFTS in Anhui province.

关 键 词：发热伴血小板减少综合征病毒 大别班达病毒 全基因组 基因型 

分 类 号：R211[医药卫生—中医学] R373