病原真菌基质辅助激光解吸电离飞行时间质谱技术鉴定准确性影响因素研究进展  

Progress in research of factors affecting accuracy of identification of pathogenic fungi by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

作　　者：陈甜甜 绳涵月 耿圆圆 张舒 赵飞[2] 金丹[1] 龚杰 Chen Tiantian;Sheng Hanyue;Geng Yuanyuan;Zhang Shu;Zhao Fei;Jin Dan;Gong Jie(Immunology Biology Key Laboratory,Yanbian University,Yanji 133002,Jilin,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;The Second Hospital of Jilin University,Changchun 130000,Jilin,China;Collaborative Innovation Center of Recovery and Reconstruction of Degraded Ecosystem in Wanjiang Basin Co-Founded by Anhui Province and Ministry of Education,School of Ecology and Environment,Anhui Normal University,Wuhu 241002,Anhui,China)

机构地区：[1]延边大学医学院免疫生物学重点实验室,吉林延吉133002 [2]中国疾病预防控制中心传染病预防控制所,传染病溯源预警与智能决策全国重点实验室,北京102206 [3]吉林大学第二医院,吉林长春130000 [4]安徽师范大学生态与环境学院,省部共建皖江流域退化生态系统的恢复与重建协同创新中心,安徽芜湖241002

出　　处：《疾病监测》2025年第2期232-238,共7页Disease Surveillance

基　　金：国家重点研发计划项目(No.2022YFC2504800);中国疾病预防控制中心青年科研基金(No.2022A102);公共卫生应急反应机制的运行-传染病防控(No.102393220020020000029)。

摘　　要：由于免疫功能低下患者的不断增多,侵袭性真菌感染(IFI)在过去几年中显著增长。IFI发病率和死亡率高,通常难以诊断和治疗。据估计,侵袭性真菌感染每年可导致约150万人死亡,死亡率超过50.00%。临床实验室中对丝状真菌的鉴定通常基于对分离培养的菌落进行形态学及分子生物学检查,这一过程耗时长且操作步骤相对复杂。基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术简单、快速、高效、通量高、成本低,弥补了临床上微生物常规鉴定的短板。目前,MALDI-TOF MS技术已经成为大多数临床实验室细菌鉴定的主流平台。然而,其在丝状真菌鉴定中的应用一直较为滞后,主要归因于缺乏真菌蛋白质提取方案的规范化工作流程,并且真菌数据库不够完善。对于病原真菌MALDI-TOF MS鉴定结果的影响因素的研究非常有限。本综述描述并分析了病原真菌MALDI-TOF MS鉴定准确性的影响因素,重点关注并比较了不同培养条件和数据库等对MALDI-TOF MS技术鉴定病原真菌的影响。The incidence of invasive fungal infection(IFI)increased significantly in the past few years due to the increase of immunocompromised patients.The morbidity and mortality of IFI are high,and the diagnosis and treatment of IFI are usually difficult.It is estimated that the mortality rate of IFI exceeds 50.00%and approximately 1.5 million deaths can be caused by IFI annually.The identification of filamentous fungi in the clinical laboratory is usually based on morphological and molecular biological examination of cultured colonies isolated,which is a slow and complex process.Matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS)is a simple,rapid,efficient,high-throughput,and low-cost method and can be used in the routine identification of microorganisms in clinical microbiology laboratories.Nowadays,MALDI-TOF MS has become the mainstream technique for bacterial identification in most clinical laboratories.However,its application in the identification of filamentous fungi is not wide,which is mainly attributed to the lack of standardized protocol for protein extraction and limited fungus databases.In addition,there is limited research on the factors affecting the accuracy of MALDI-TOF MS identification of pathogenic fungi.This paper describes and analyzes the factors affecting the accuracy of MALDI-TOF MS identification of pathogenic fungi,focusing on the impacts of different culture conditions and databases.

关 键 词：侵袭性真菌感染 基质辅助激光解吸电离飞行时间质谱技术 培养条件 数据库 

分 类 号：R211[医药卫生—中医学] R37