FEA对PRV体内外感染诱导免疫细胞组蛋白乙酰化修饰的调节作用  

Regulatory effect of FEA on histone acetylation modification in immune cells induced by PRV infection in vivo and in vitro

作　　者：余小丽 袁海峰 黄昌巧 蒋丽群 李艳华 胡效东 任春芝 胡庭俊[1] YU Xiaoli;YUAN Haifeng;HUANG Changqiao;JIANG Liqun;LI Yanhua;HU Xiaodong;REN Chunzhi;HU Tingjun

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]徐州生物工程职业技术学院,江苏徐州221006

出　　处：《饲料研究》2025年第4期56-61,共6页Feed Research

基　　金：国家自然科学基金项目(项目编号:32072907);广西大学巴马产教融合研究院人才科技专项(项目编号:20220024);南宁市科学研究与技术开发计划项目(项目编号:20232039);广西研究生教育创新计划项目(项目编号:JGY2023015);广西大学-大学生创新创业训练计划(项目编号:S202310593294)。

摘　　要：研究旨在观察辣蓼黄酮乙酸乙酯部分(FEA)对猪伪狂犬病毒(PRV)感染小鼠和细胞组蛋白乙酰化修饰的调节作用。试验包括体内试验和体外试验两部分。体外试验设细胞对照组、曲古霉素A(TSA)组、PRV组、芦丁组及PRV+FEA 25组、PRV+FEA 50组、PRV+FEA 100组。体内试验设空白对照组、TSA组、PRV组、芦丁组、PRV+FEA 50组、PRV+FEA 100组、PRV+FEA 200组。测定样品乙酰转移酶1(HAT1)、去乙酰转移酶1(HDAC1)的活性,HAT1和HDAC1的mRNA表达水平及组蛋白H3(AcH3)和H4(AcH4)乙酰化水平。结果显示,与PRV组相比,FEA组RAW264.7细胞HAT1的活性均极显著降低(P<0.01),PRV+FEA 25组、PRV+FEA 50组HDAC1的活性显著升高(P<0.05);PRV+FEA 50组、PRV+FEA 100组HAT1 mRNA的表达水平极显著降低(P<0.01),PRV+FEA 25组、PRV+FEA 50组HDAC1 mRNA表达水平显著提高(P<0.05);PRV+FEA 25组、PRV+FEA 100组AcH3乙酰化水平及PRV+FEA 50组、PRV+FEA 100组AcH4乙酰化水平极显著降低(P<0.01),PRV+FEA 50组AcH3乙酰化水平显著降低(P<0.05)。与PRV组相比,PRV+FEA 100组、PRV+FEA 200组小鼠脾脏细胞HAT1活性极显著降低(P<0.01),而HDAC1活性极显著提升(P<0.01);PRV+FEA 100组、PRV+FEA 200组小鼠脾脏HAT1 mRNA的表达水平极显著降低(P<0.01),PRV+FEA 50组小鼠脾脏HDAC1 mRNA的表达水平显著升高(P<0.05),PRV+FEA 100组、PRV+FEA 200组小鼠脾脏HDAC1 mRNA的表达水平极显著升高(P<0.01);PRV+FEA 50组、PRV+FEA 100组小鼠脾脏AcH3乙酰化表达水平以及PRV+FEA 100组、PRV+FEA 200组小鼠脾脏AcH4乙酰化表达水平极显著降低(P<0.01),PRV+FEA 200组小鼠脾脏AcH3乙酰化表达水平显著降低(P<0.05)。研究表明,FEA能够调控PRV体内外感染诱导的免疫细胞组蛋白乙酰化修饰水平。The study aimed to observe the regulatory effect of the ethyl acetate fraction of Fagopyrumflavonoids(FEA)on histone acetylation modification in mice and cells infected with pseudorabies virus(PRV).The experiment consisted of invivoand invitroparts.In the invitroexperiment,there were cell control group,trichostatin A(TSA)group,PRV group,rutin group,PRV+FEA 25 group,PRV+FEA 50 group,and PRV+FEA 100 group.In the invivo experiment,there were blank control group,TSA group,PRV group,rutin group,PRV+FEA 50 group,PRV+FEA 100 group,and PRV+FEA 200 group.The enzyme activities of histone acetyltransferase 1(HAT1)and histone deacetylase 1(HDAC1),the mRNA expression levels of HAT1 and HDAC1,and the acetylation levels of histone H3(AcH3)and H4(AcH4)were measured.The results showed that compared with the PRV group,the activity of HAT1 in RAW264.7 cells in the FEA groups was extremaly decreased(P<0.01),and the activity of HDAC1 in the PRV+FEA 25 group and PRV+FEA 50 group was significantly increased(P<0.05).The mRNA expression level of HAT1 in the PRV+FEA 50 group and PRV+FEA 100 group was extremely decreased(P<0.01),while the mRNA expression level of HDAC1 in the PRV+FEA 25 group and PRV+FEA 50 group was significantly increased(P<0.05),The acetylation level of AcH3 in the PRV+FEA 25 group and PRV+FEA 100 group,and the acetylation level of AcH4 in the PRV+FEA 50 group and PRV+FEA 100 group were extremely decreased(P<0.01),and the acetylation level of AcH3 in the PRV+FEA 50 group was significantly decreased(P<0.05).Compared with the PRV group,the activity of HAT1 in the splenocytes of mice in the PRV+FEA 100 group and PRV+FEA 200 group was extremely decreased(P<0.01),while the activity of HDAC1 was extremely increased(P<0.01).The mRNA expression level of HAT1 in the spleen of mice in the PRV+FEA 100 group and PRV+FEA 200 group was extremely decreased(P<0.01),the mRNA expression level of HDAC1 in the spleen of mice in the PRV+FEA 50 group was significantly increased(P<0.05),and the mRNA expression level of HDAC1 in the spleen of

关 键 词：辣蓼黄酮乙酸乙酯部分 伪狂犬病毒 组蛋白乙酰化 

分 类 号：S816.7[农业科学—饲料科学]