响应面优化杜仲苷超声辅助提取工艺及其抗氧化活性研究  

Study on optimization of ultrasonic assisted extraction process and antioxidant activity of Eucommia ulmoides glycosides using response surface methodology

作　　者：曹雨欣 张雪蕾 赵传生 毕珂彤 王松山 张萌 吕培凡 张勇[1] CAO Yuxin;ZHANG Xuelei;ZHAO Chuansheng;BI Ketong;WANG Songshan;ZHANG Meng;LÜPeifan;ZHANG Yong

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《饲料研究》2025年第5期73-77,共5页Feed Research

基　　金：河南省高等学校重点科研项目(项目编号:24B230001);河南工业大学高层次人才引进项目(项目编号:2022BS072)。

摘　　要：试验旨在优化杜仲苷的提取工艺并探究其抗氧化活性。采用超声辅助水提法,以杜仲叶为原料,以液料比、超声温度和超声时间为自变量,以杜仲苷提取率为响应值,通过响应面分析法优化提取工艺参数,并对杜仲苷的微观结构及抗氧化活性进行研究。结果表明,在液料比19 mL/g、超声温度69℃、超声时间39 min的条件下,杜仲苷提取率最高,为7.22%。杜仲苷表面粗糙,具有明显的空洞结构。当杜仲苷质量浓度为3.0 g/L时,其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟基自由基和2,2-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基的清除率分别为90.06%、77.62%和100.00%。研究表明,杜仲苷可以作为抗氧化饲料添加剂应用于动物养殖生产中。The experiment aimed to optimize the extraction process of Eucommia ulmoides glycosides and explore its antioxidant activity.Using ultrasound assisted water extraction method,Eucommia ulmoides leaves were used as raw materials,with liquid to material ratio,ultrasound temperature,and ultrasound time as independent variables,and Eucommia ulmoides glycosides extraction rate as the response value.The extraction process parameters were optimized by response surface analysis,and its microstructure and antioxidant activity were studied.The results showed that under the conditions of liquid-solid ratio of 19 mL/g,ultrasonic temperature of 69℃and ultrasonic time of 39 min,the extraction yield of Eucommia ulmoides glycosides was the highest(7.22%).The surface of Eucommia ulmoides glycosides was rough and had obvious void structure.The scavenging efficiency of 1,1-diphenyl-2-trinitrophenylhydrazide(DPPH)free radical,hydroxyl free radical and 2,2-diazo-bis(3-ethyl-benzothiazolin-6-sulfonic acid)diamiammonium salt(ABTS)free radical were 90.06%,77.62%and 100.00%,respectively,when the concentration of Eucommia ulmoides glycosides was 3.0 g/L.The study indicates that Eucommia ulmoides glycosides can be used as antioxidant feed additive in animal breeding.

关 键 词：杜仲苷 提取工艺 响应面分析法 抗氧化 

分 类 号：S816.7[农业科学—饲料科学]