Bioinspired Surface Engineering with Dual Covalent Receptors Incorporated via Precise Post-Imprinting Modification to Enhance the Specific Identification of Adenosine 5′-Monophosphate  

作　　者：Pan Wang Tao Cheng Zhuangxin Wei Lu Liu Yue Wang Xiaohua Tian Jianming Pan 

机构地区：[1]School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China

出　　处：《Engineering》2025年第2期143-154,共12页工程(英文)

基　　金：supported by the National Natural Science Foundation of China(22078132,22108103,and U22A20413);the Open Funding Project of the National Key Labora-tory of Biochemical Engineering(2021KF-02);China Postdoctoral Science Foundation(2021M691301);Jiangsu Key Research and Development Program(BE2022356);the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation(CPSF)(GZ20230989);Jiangsu Agricultural Independent Innovation Fund Project(CX(21)3079).

摘　　要：Expanding the specific surface area of substrates and carrying out precise surface engineering of imprinted nanocavities are crucial methods for enhancing the identification efficiency of molecularly imprinted polymers(MIPs).To implement this synergistic strategy,bioinspired surface engineering was used to incorporate dual covalent receptors via precise post-imprinting modifications(PIMs)onto mesoporous silica nanosheets.The prepared sorbents(denoted as‘‘D-PMIPs”)were utilized to improve the specific identification of adenosine 5-monophosphate(AMP).Significantly,the mesoporous silica nanosheets possess a high surface area of approximately 498.73 m^(2)·g^(-1),which facilitates the formation of abundant specific recognition sites in the D-PMIPs.The dual covalent receptors are valuable for estab-lishing the spatial orientation and arrangement of AMP through multiple cooperative interactions.PIMs enable precise site-specific functionalization within the imprinted cavities,leading to the tailor-made formation of complementary binding sites.The maximum number of high-affinity binding sites(Nmax)of the D-PMIPs is 39.99 lmol·g^(-1),which is significantly higher than that of imprinted sorbents with a sin-gle receptor(i.e.,S-BMIPs or S-PMIPs).The kinetic data of the D-PMIPs can be effectively described by a pseudo-second-order model,indicating that the main binding mechanism involves synergistic chemisorption from boronate affinity and the pyrimidine base.This study suggests that using dual cova-lent receptors and PIMs is a reliable approach for creating imprinted sorbents with high selectivity,allow-ing for the controlled engineering of imprinted sites.

关 键 词：Precise surface engineering Dual covalent receptor Precise post-imprinting modification Specific identification of adenosine 5-monophosphate 

分 类 号：O657.7[理学—分析化学] O657.3[理学—化学]