长毛兔PRKACB基因多态性及其与产毛量的关联分析  

Polymorphism of PRKACB Gene and Its Correlation with Wool Yield in Angora Rabbits

作　　者：任宇尧 韩旭东 刘燕[2] 赵博昊 陈阳[1] 吴信生[1] REN Yuyao;HAN Xudong;LIU Yan;ZHAO Bohao;CHEN Yang;WU Xinsheng(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009;Institute of Animal Hubandry and Veterinary Sciences,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]浙江省农科院畜牧兽医研究所,浙江杭州310021

出　　处：《家畜生态学报》2025年第3期9-14,共6页Journal of Domestic Animal Ecology

摘　　要：本试验旨在分析长毛兔PRKACB基因序列遗传多态性及其与生产性状(体重、产毛量)的相关性,发现与产毛量相关的分子标记,为加快毛兔选育提供参考。试验采用直接测序法检测了169只皖系长毛兔PRKACB基因外显子区与启动子区序列的SNPs位点,进行遗传多样性分析,并运用SPSS 25.0软件进行SNPs与体重、产毛量的关联性分析。结果显示,长毛兔PRKACB共有10个外显子和9个内含子,其中外显子序列较为保守,未检测到SNPs。启动子序列(1366 bp)存在2个SNP位点(rs81065607A>C和rs81066149C>T),均包含3种基因型,均符合哈迪-温伯格平衡。rs81065607A>C和rs81066149C>T的PIC分别为0.331和0.327,均为中度多态,提示PRKACB基因在群体中具有较高遗传变异能力。SNP位点间连锁不平衡程度结果显示,2个位点高度连锁(r^(2)=0.983)。关联分析表明,rs81065607A>C的CC型个体的产毛量显著高于AA型和AC型(P<0.05);rs81066149C>T的TT型个体的产毛量显著高于CC型和CT型(P<0.05)。结果表明,PRKACB基因rs81065607A>C和rs81066149C>T位点可以作为长毛兔产毛量的潜在分子标记,用于长毛兔的分子选育。The objective of this study was to analyze the genetic polymorphism of PRKACB gene sequence in Angora rabbits and its correlation with production traits(body weight,wool yield),and to identify molecular markers in relation to wool production,and to provide reference for accelerating the breeding of Angora rabbits.Direct sequencing was used to detect SNPs in the PRKACB gene exon and promoter region of 169 Angora rabbits,and SPSS 25.0 software was used to analyze the correlation between SNPs and weight and wool production.Results showed PRKACB gene has 10 exons and 9 introns,with the exon sequences being relatively conserved and no SNPs detected.In the 1366bp promoter sequence,two SNP loci(rs81065607A>C and rs81066149C>T)were found,both containing three genotypes and conforming to Hard-Weinberg equilibrium.The polymorphic information content values of rs81065607A>C and rs81066149C>T were 0.331 and 0.327,indicating moderate polymorphism,suggesting that the PRKACB gene has high genetic variability in the Angora rabbit population.The linkage disequilibrium analysis showed a high linkage between the two SNP loci(r^(2)=0.983).The association analysis showed that the individuals with the CC genotypes of rs81065607A>C had significantly higher wool production than those with AA and AC genotypes(P<0.05);individuals with the TT genotype of rs81066149C>T had significantly higher wool yield than those with CC and CT genotypes(P<0.05).It is therefore concluded that the SNP sites rs81065607A>C and rs81066149C>T in the PRKACB gene can be potential molecular markers for wool yield in Angora rabbits,and can be used in molecular breeding of Angora rabbits.

关 键 词：长毛兔 PRKACB基因 产毛量 SNP 关联分析 

分 类 号：S829.1[农业科学—畜牧学]