云南斑籽木化学成分及其抗炎活性研究  

Chemical Constituents of Baliospermum calycinum and Their Anti-inflammatory Activities

作　　者：李怡欢 卢光衣 巴翌航 何鑫然 何红平 蒋华夷 LI Yi-huan;LU Guang-yi;BA Yi-hang;HE Xin-ran;HE Hong-ping;JIANG Hua-yi(School of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;College of Medicine,Yunnan College of Business Management,Kunming 650101,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]云南经济管理学院医学院,云南昆明650101

出　　处：《中药材》2024年第8期1951-1956,共6页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金资助项目(82260839);云南省应用基础研究计划项目(202001AU070104,202301AT070250);云南省教育厅科学研究基金资助项目(2023Y0460)。

摘　　要：目的:研究大戟科斑籽木属植物云南斑籽木Baliospermum calycinum Müll.Arg.的化学成分及其体外抗炎活性。方法:结合硅胶柱色谱、RP-C18柱色谱、Sephadex LH-20葡聚糖凝胶柱色谱、半制备HPLC等方法对云南斑籽木的95%乙醇提取物进行分离纯化,根据理化性质及波谱数据鉴定化合物结构。通过LPS诱导的RAW 264.7巨噬细胞炎症模型对化合物进行抗炎活性筛选。结果:从云南斑籽木中分离得到20个化合物,分别鉴定为:ajugamarin B2(1)、ajuganipponin A(2)、cassipouryl acetate(3)、反式-植物醇(4)、3-羟基-7,8-二脱氢-β-紫罗兰酮(5)、3-羟基-5α,6α-环氧-β-紫罗兰酮(6)、3-羟基-β-紫罗兰酮(7)、9-hydroxy-4,7-megastigmadien-3-one(8)、去氢吐叶醇(9)、9-hydroxy-4-megastigmen-3-one(10)、吐叶醇(11)、蚱蜢酮(12)、黑麦草内酯(13)、pubinernoid A(14)、吲哚-3-甲醛(15)、吲唑(16)、欧芹酚甲醚(17)、东莨菪内酯(18)、异嗪皮啶(19)、楝叶吴萸素B(20)。化合物5在25μmol/L浓度下能够抑制LPS诱导的RAW 264.7巨噬细胞内NO的释放,IC50值为(18.44±0.76)μmol/L。结论:其中,化合物2、3、5~8、10、12、16~20为首次从斑籽木属植物中分离得到,其他化合物为首次从该植物中分离得到。化合物5具有潜在的抗炎活性。Objective:To study the chemical constituents and in vitro anti-inflammatory activities of Baliospermum calycinum.Methods:The 95%ethanol extract of Baliospermum calycinum was isolated and purified by silica gel column chromatography,RP-C18 column chromatography,Sephadex LH-20 gel column chromatography and semi-HPLC,the structures of the compounds were identified according to their physical and chemical properties and spectral data.The LPS-induced RAW 264.7 macrophage inflammation model was used to determine the anti-inflammatory activities of the compounds.Results:Twenty compounds were isolated from Baliospermum calycinum and identified as ajugamarin B2(1),ajuganipponin A(2),cassipouryl acetate(3),trans-phytol(4),3-hydroxy-7,8-didehydro-β-ionone(5),3-hydroxy-5α,6α-epoxy-β-ionone(6),3-hydroxy-β-ionone(7),9-hydroxy-4,7-megastigmadien-3-one(8),dehydrovomifoliol(9),9-hydroxy-4-megastigmen-3-one(10),vomifoliol(11),grasshopper ketone(12),loliolide(13),pubinernoid A(14),indole-3-carboxal-dehyde(15),indazole(16),osthol(17),scopoletin(18),isofraxidin(19),evofolin B(20),respectively.Compound 5 inhibited LPS-induced NO release from RAW 264.7 macrophages at a concentration of 25μmol/L with IC50 value of(18.44±0.76)μmol/L.Conclusion:Among them,compounds 2,3,5 to 8,10,12,16 to 20 are isolated from Baliospermum genus for the first time.Other compounds are isolated from Baliospermum calycinum for the first time.Compound 5 shows potential anti-inflammatory activity.

关 键 词：云南斑籽木 化学成分 分离鉴定 抗炎 

分 类 号：R284.1[医药卫生—中药学]