内生真菌皮壳青霉化学成分研究  

Chemical Constituents of the Endophytic Fungus Penicillium crustosum

作　　者：林冬梅 杨莲 李妃杏 李小龙 杨瑾 杨晓艳[1] LIN Dong-mei;YANG Lian;LI Fei-xing;LI Xiao-long;YANG Jin;YANG Xiao-yan(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《中药材》2024年第8期1981-1985,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金项目(32060104)。

摘　　要：目的:研究内生真菌皮壳青霉Penicillium crustosum固体发酵产物的化学成分。方法:利用硅胶、Sephadex LH-20葡聚糖凝胶、高效液相色谱等多种色谱分离技术对皮壳青霉固体发酵产物的乙酸乙酯部位进行分离,通过NMR和ESI-MS鉴定化合物结构,通过二倍稀释法和CCK-8法对化合物进行抑菌及抗肿瘤活性测试。结果:从皮壳青霉发酵产物中分离纯化得到12个化合物,分别鉴定为:2′,4′-dihydroxy-3′,5′-dimethyl acetophenone(1)、4-methyl-6-acetylresorcinol(2)、methyl-2,4-dihydroxy-3,5,6-trimethylbenzoate(3)、communol F(4)、communol E(5)、6-acetyl-2α,5-dihydroxy-2-(2-hydroxypropyl)-3α,8-dimethylchroman(6)、butyl isobutyl phthalate(7)、ethyl ferulate(8)、7βhydroxygeosmin(9)、3-hydroxy-4-phenylquinolin-2(1H)-one(10)、(Z)-3-benzylidene-4-methyl-3,4-dihydro-1H-benzo[e][1,4]diazepine-2,5-dione(11)、(3S)-1,4-benzodiazepine-2,5-diones(12)。化合物的MIC值均大于100μg/mL,IC50值均大于100μmol/L。结论:其中,化合物2、4、5、7~9为首次从皮壳青霉中分离得到。所有化合物均未表现出明显的抑菌或抗肿瘤活性。Objective:To study the chemical constituents of the solid fermentation products of the endophytic fungus Penicillium crustosum.Methods:Silica gel,Sephadex LH-20,HPLC and other chromatographic separation technologies were used to isolate the ethyl acetate part of the solid fermentation product of Penicillium crustosum.The structures were identified by NMR and ESI-MS.Their antibacterial and anti-tumor activities were tested by double dilution method and CCK-8 method.Results:Twelve compounds were isolated and purified from the fermentation products of Penicillium crustosum and identified as 2′,4′-dihydroxy-3′,5′-dimethyl acetophenone(1),4-methyl-6-acetylresorcinol(2),methyl-2,4-dihydroxy-3,5,6-trimethylbenzoate(3),communol F(4),communol E(5),6-acetyl2α,5-dihydroxy-2-(2-hydroxypropyl)-3α,8-dimethylchroman(6),butyl isobutyl phthalate(7),ethyl ferulate(8),7β-hydroxygeosmin(9),3-hydroxy-4-phenylquinolin-2(1H)-one(10),(Z)-3-benzylidene-4-methyl-3,4-dihydro-1H-benzo[e][1,4]diazepine-2,5-dione(11),(3S)-1,4-benzodiazepine-2,5-diones(12),respectively.The MIC values of the compounds were all greater than 100μg/mL,and the IC50 values were all greater than 100μmol/L.Conclusion:Among them,compounds 2,4,5,7 to 9 are isolated from Penicillium crustosum for the first time,none of the compounds shows significant antibacterial or anti-tumor activity.

关 键 词：皮壳青霉 内生真菌 化学成分 生物活性 

分 类 号：R284.1[医药卫生—中药学]