雌雄土鳖虫的纤溶和抗凝活性对比研究  

Comparative study on the activities of fibrinolysis and antithrombosis in male and female ground beetle

作　　者：曾以旺 ZENG Yiwang(Department of Pharmacy,Ganzhou People’s Hospital,Ganzhou 341000,Jiangxi,China)

机构地区：[1]赣州市人民医院药剂科,江西赣州341000

出　　处：《中国现代医生》2025年第10期55-58,共4页China Modern Doctor

摘　　要：目的比较雌雄土鳖虫的纤溶和抗凝活性。方法采用回流方式对雌雄土鳖虫药材进行提取,进行体外纤溶实验活性评价;通过注射盐酸肾上腺素和强制冰水游泳构建寒凝血瘀小鼠模型,将小鼠分为空白组、模型组、尿激酶组、雄土鳖虫高和低剂量组、雌土鳖虫高和低剂量组,每组10只。测定全血黏度、血细胞等指标评价雌雄土鳖虫抗凝活性。结果体外纤溶实验结果显示雌、雄土鳖虫的低、高剂量组体外纤溶活性比较差异均无统计学意义(P>0.05)。模型组小鼠的全血黏度、血细胞比容、血小板计数均显著高于空白组(P<0.05)。雌、雄土鳖虫的低、高剂量组小鼠低切变率的全血黏度、血细胞比容、血小板计数均显著低于模型组(P<0.05)。结论雌、雄土鳖虫在纤溶和抗凝活性方面并无显著差异,这为雌、雄土鳖虫的进一步开发利用提供支撑。Objective To compare the activities of fibrinolysis and antithrombosis between male and female ground beetle.Methods The medicinal materials of male and female ground beetle were extracted by reflux method,and the fibrinolytic activity was evaluated in vitro.The mouse model of cold congelation and blood stasis was established by injection of epinephrine hydrochloride and forced swimming in ice water.The mice were divided into blank group,model group,urokinase group,high and low dose male ground beetle group,high and low dose female ground beetle group,with 10 mice in each group.The anticoagulant activity of male and female ground beetle was evaluated by measuring whole blood viscosity and blood cells.Results The results of in vitro fibrinolysis test showed that there was no significant difference in fibrinolytic activity between the low and high dose groups of male and female ground beetle(P>0.05).The whole blood viscosity,hematocrit,and platelet count of model group were significantly higher than those of blank group(P<0.05).The low shear rate of whole blood viscosity,hematocrit and platelet count of low and high dose groups male and female ground beetle were significantly lower than those in model group(P<0.05).Conclusion There was no significant difference in fibrinolytic and anticoagulant activities between male and female ground beetle,which provided support for the further development and utilization of male and female ground beetle.

关 键 词：体外纤溶实验 寒凝血瘀 雌雄土鳖虫 活血化瘀 

分 类 号：R285.5[医药卫生—中药学]