‘贵紫麦1号’GzPIN1-7B基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of GzPIN1-7B Gene from of'Guizimai No.1'

作　　者：熊富敏 李鲁华 梁涛 安畅 徐向军 任明见[1,2] 徐如宏[1,2] Xiong Fumin;Li Luhua;Liang Tao;An Chang;Xu Xiangjun;Ren Mingjian;Xu Ruhong(College of Agriculture,Guizhou University,Guiyang,550025;Guizhou Subcenter of National Wheat Improvement Center,Guiyang,550025)

机构地区：[1]贵州大学农学院,贵阳550025 [2]国家小麦改良中心贵州分中心,贵阳550025

出　　处：《分子植物育种》2025年第3期792-802,共11页Molecular Plant Breeding

基　　金：贵州省科技支撑计划项目(黔科合支撑[2021]一般272);贵州省农业成果转化计划项目(黔科合成果[2019]4246号;黔科合平台人才[2017]5788号);贵州省科技计划项目(黔科合基础[2020]1Z018号;黔科合基础[2019]1073号)共同资助。

摘　　要：为了解小麦生长素外输基因PIN1的基本结构和生物学功能。本研究以‘贵紫麦1号’的cDNA序列全长为模板,通过RT-PCR方法得到GzPIN1-7B基因,利用生物信息学分析软件对该基因及其编码的蛋白质进行分析。结果表明:GzPIN1-7B蛋白共编码586个氨基酸,属于疏水性蛋白,亚细胞定位在细胞膜上;该蛋白不存在信号肽,是一种分泌蛋白;蛋白功能结构域分析显示其含有两个Mem-trans结构域,属于Mem-trans超家族;二、三级结构预测显示主要由无规卷曲和α-螺旋组成;其编码的氨基酸序列与大麦、小麦、节节麦的同源关系较近;其启动子顺式作用元件分析结果显示,GzPIN1-7B启动子中除了含有CAAT-box和TATA-box等核心启动子元件外,还有响应激素和逆境胁迫等相关的顺式作用元件。以上研究结果为进一步探索GzPIN1-7B基因参与小麦生长发育的调控机制提供理论依据。In order to study the basic structure and biological functions of wheat auxin transmission gene PIN1.In this study,GzPIN1-7B gene was obtained by RT-PCR with the full-length cDNA sequence of'Guizimai No.1'as a template,and the gene and its encoded protein was analyzed by using bioinformatics analysis software.The results showed that:GzPIN1-7B protein encoding 586 amino acids,belonging to the hydrophobic protein,sub-cellular localization in the cell membrane,which is a kind of secretory proteins without signal peptide.Function structure domain analysis showed that it belonged to the Mem-trans super family,and contains two conserved Mem-trans domains.Secondary and tertiary structures analysis showed it was mainly composed of random coil and alpha helix.The homologous of amino acid was close to Hordeum vulgare L.and Triticum aestivum L.and A egilops tauschii subsp..Promoter cis-element analysis indicated that GzPIN1-7B not only contains CAAT box and TATA box promoter elements,but also contains hormone and adversity stress cis element.The above results can provide theoretical basis for further explore GzPIN1-7B gene participate in the regulatory mechanism of wheat growth and development.

关 键 词：‘贵紫麦1号’ GzPIN1-7B 基因克隆 生物信息学分析 

分 类 号：S512.1[农业科学—作物学]