低温胁迫下高粱的数字基因表达谱分析  

Digital Gene Expression Profiling Analysis of Sorghum under Low Temperature Stress

作　　者：周福平[1] 王晓清 张海燕 史红梅 杨彬 闫凤霞 Zhou Fuping;Wang Xiaoqing;Zhang Haiyan;Shi Hongmei;Yang Bin;Yan Fengxia(Key Laboratory of Genetic and Germplasm Innovation in Sorghum for Shanxi Province,Institute of Sorghum,Shanxi Agricultural University,Jinzhong,030600;College of Life Sciences,Shanxi Agricultural University,Taiyuan,030031)

机构地区：[1]山西农业大学高粱研究所,高粱遗传与种质创新山西省重点实验室,晋中030600 [2]山西农业大学生命科学学院,太原030031

出　　处：《分子植物育种》2025年第3期803-816,共14页Molecular Plant Breeding

基　　金：山西农业大学生物育种工程项目(YZGC061)资助。

摘　　要：本研究旨在发掘高粱在低温胁迫响应过程中的关键调控基因,探讨高粱在低温胁迫下的耐受机制,从而有针对性地选择抗冷亲本,并为高粱耐冷分子育种提供参考。以耐冷高粱品系‘红壳四号’及冷敏感高粱品系‘SC407’为研究材料,利用数字基因表达谱(DGE)技术对低温胁迫(2℃)后的高粱幼苗植株cDNA文库中的表达基因进行分析注释。结果表明,两个品系共有5763个基因受低温胁迫诱导表达。GO功能显著性富集分析表明,两个品系的差异表达基因主要涉及细胞过程、单组织过程、代谢过程、结合功能以及催化活性。KEGG富集分析表明,两个品系的差异基因主要富集在苯丙烷类合成、碳代谢、氨基酸生物合成、植物激素信号转导及淀粉与蔗糖代谢途径等5条途径;冷敏品系中差异基因主要集中在代谢途径,冷抗品系中差异基因主要集中在修复和抗氧化物质的合成以及植物激素信号转导与谷胱甘肽代谢途径。这些发现将有助于进一步理解高粱的冷胁迫应答机制。高粱在低温胁迫下,可通过基因表达的诱导和抑制来提高植物体本身的耐冷能力。同时,高粱的耐冷机制调控是一个复杂的过程,是由不同通路一系列基因表达共同作用的结果。本研究可为高粱耐冷分子育种提供信息支持。This research aims to explore the key regulatory genes in response to low temperature stress in sorghum,explore the tolerance mechanism of sorghum under low temperature stress,so as to select cold resistant parents and provide reference for molecular breeding of cold tolerance in sorghum.Using cold-tolerant sorghum line'Hongke4'and cold-sensitive sorghum line'SC407'as research materials,differential gene expression profiles of sorghum leaf cDNA libraries treated by low temperature stress(2℃)were analyzed by digital gene expression profiling(DGE).A total of 5763 genes were differentially expressed under low temperature stress in the two lines.Significant enrichment analysis of GO function showed that DEGs of the two lines mainly involved cell process,single tissue process,metabolic process,binding function and catalytic activity.KEGG enrichment analysis showed that the differential genes of the two lines were mainly concentrated on five pathways:phenylpropane synthesis,carbon metabolism,amino acid biosynthesis,phytohormone signal transduction and starch and sucrose metabolism pathway;the differential genes of cold-sensitive were mainly concentrated on metabolic pathway;the differential genes of cold-resistant lines were mainly concentrated on repair and antioxidant synthesis and phytohormone signal transduction and glutathione metabolism pathway.These findings will help us further understand the mechanism of sorghum cold stress response.Sorghum can improve its cold tolerance under low temperature stress by inducing and inhibiting gene expression,and the regulation of sorghum on cold tolerance mechanism of low temperature is a complex process,which is the result of a series of gene expressions in different pathways.This study can provide information support for sorghum cold tolerance molecular breeding.

关 键 词：高粱 低温胁迫 转录组测序 差异表达基因 

分 类 号：S514[农业科学—作物学]