续随子CYP726A30基因克隆及生物信息学分析  

Gene Cloning and Bioinformatics Analysis of CY P726A 30 from Euphorbia lathyris L.

作　　者：彭健 田梅 赵万里 王帆 林谷音 冯煦[1] 陈雨[1] Peng Jian;Tian Mei;Zhao Wanli;Wang Fan;Lin Guyin;Feng Xu;Chen Yu*(The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm,Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Zhongshan Botanical Garden),Nanjing,210014)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏省植物资源研究与利用重点实验室,江苏省农业种质资源保护与利用平台,南京210014

出　　处：《分子植物育种》2025年第3期955-962,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31770383,31970375);江苏省自然科学基金项目(BK20200295);中国博士后科学基金项目(2020M681527)共同资助。

摘　　要：从续随子转录组数据中挖掘到一个新的CYP726A亚家族的萜类合成酶,命名为ElCYP726A30。本研究将其序列成功克隆至载体,并对其进行生物信息学分析、组织特异性表达分析。结果表明,ElCYP726A30基因开放阅读框(ORF)包含1479个碱基,编码493个氨基酸。ElCYP726A30的理论相对分子质量为56.07 kD,等电点8.75。该基因表达的蛋白是一种亲水稳定蛋白质,C段有信号肽、无跨膜区,可能定位于内质网膜上。ElCYP726A30蛋白二级结构主要为α-螺旋,占48.88%;其次为无规卷曲、延伸链和β-转角,分别占据32.86%、12.37%和5.88%。多重序列比对和聚类分析表明,续随子ElCYP726A30蛋白与同科植物南欧大戟的CYP726A6蛋白氨基酸序列高度同源。组织特异性表达分析结果显示ElCYP726A30在续随子根中表达量最高。A new C YP726A subfamil y P450 terpene synthase,named as ElCYP726A30,was mined from the transcriptome data of Euphorbia lathyris L..This study aims to clone the cDNA sequence of ElCYP726A30 in E.lathyris L.,perform bioinformatics analysis and tissue specific expression analysis of ElCYP726A30.The results showed that the open reading frame(ORF)of ElCY P726A 30 gene contained 1479 bases and encoded 493 amino acids.The theoretical relative molecular weight of ElCYP726A30 was 56.07 kD and the isoelectric point was 8.75.ElCYP726A30 was a hydrophilic and stable protein with signal peptide in C-terminus,no transmembrane region and might be located on the endoplasmic reticulum.The secondary structure of ElCYP726A30 was dominated byα-helix,accounting for 48.88%,followed by random coil,extended strand andβ-rotation,accounting for 32.86%,12.37%and 5.88%,respectively.Multiple sequence alignment and cluster analysis showed that ECYP726A30 was highly similar to CYP726A6 protein sequence from Euphorbia peplus.Tissue specific expression analysis indicated that ElCY P726A 30 had the highest expression level in roots of E.lathyris L..

关 键 词：续随子(Euphorbia lathyris L.) 细胞色素P450酶 基因克隆 生物信息学分析 

分 类 号：Q943.2[生物学—植物学]