Gadd45α基因的敲除对胚胎干细胞基因组稳定性的影响  

Effect of Gadd45αKnockout on Genomic Stability in Embryonic Stem Cells

作　　者：李冲 吴昊 龙琪 Li Chong;Wu Hao;Long Qi(College of Pharmacy,Nankai University,Tianjin 300350,China;Center for Cell Lineage and Atlas,Guangzhou 510000,China;College of Life Sciences,Guangzhou Medical University,Guangzhou 510000,China)

机构地区：[1]南开大学药学院,天津300350 [2]生物岛实验室细胞谱系研究中心,广东广州510000 [3]广州医科大学生命科学学院,广东广州510000

出　　处：《南开大学学报(自然科学版)》2025年第1期73-77,共5页Journal of Nankai University(Natural Sience)

基　　金：广东省自然科学基金面上项目(2020A1515010919)。

摘　　要：基因组的稳定性是细胞完成各项生物学功能的前提,Gadd45α蛋白与DNA修复、细胞周期调节和应激反应等多个生物学过程相关.由于Gadd45α参与了DNA损伤修复,其可能和基因组的稳定性存在着密切联系;通过免疫荧光实验发现了Gadd45α分布于染色质周围,可能与染色质结构相关;进一步利用CRISPR/Cas9基因编辑技术,构建了Gadd45α基因敲除的小鼠胚胎干细胞系,通过彗星电泳,发现Gadd45α基因的敲除破坏了胚胎干细胞基因组的稳定性.初步探索了Gadd45α蛋白的功能,为进一步研究Gadd45家族在DNA损伤及修复中的作用提供了研究基础.Keeping the genomic stability is essential for cells in development and growth.Gadd45α plays an important role in DNA repair,cell cycle regulation and stress response.It may also be a key factor in keeping the genomic stability.The distribution of Gadd45α around heterochromatin was discovered through immunofluorescence,indicating its role in maintaining genomic stability.To further study the correlation of Gadd45α and genomic stability,the Gadd45α gene knockout mouse embryonic stem cell line by CRISPR/Cas9 system was constructed and identified.Comet assay showed that the genomic stability was weaker by Gadd45α knockout.Our work would benefit the study of Gadd45 family proteins in DNA damage and repair.

关 键 词：Gadd45α蛋白 DNA损伤修复 CRISPR/Cas9 基因组稳定性 

分 类 号：Q71[生物学—分子生物学]