低温胁迫下军曹鱼幼鱼肝脏转录组测序分析  

Transcriptome analysis of liver of juvenile Rachycentron canadum under low temperature stress

作　　者：汤保贵[1,2] 蔡润佳 张静[1,2] Tang Baogui;Cai Runjia;Zhang Jing(Fishery College,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东省水产经济动物病原生物学及流行病学重点实验室,广东湛江524088

出　　处：《海洋学报》2025年第1期63-73,共11页

基　　金：南方海洋科学与工程广东省实验室(湛江)项目(ZJW-2019-06);湛江市海洋经济创新发展示范市项目(XM-202008-03);国家海水鱼产业技术体系项目(CARS-47-G08)。

摘　　要：为研究低温胁迫对军曹鱼幼鱼的影响,将军曹鱼幼鱼饲养于常温[(30.5±1.0)℃]和低温[(20.0±0.5)℃]7 d后对军曹鱼肝脏进行有参转录组测序,每组3个生物学重复。结果显示,6个测序样品共检测到约243694134个raw reads,所有样品Q30均超过94%,GC在47.65%~48.16%范围内。共筛选出4362个差异表达基因,其中2793个基因上调,1569个基因下调。KEGG通路富集分析结果显示,在脂质代谢中,大量差异基因富集在脂代谢过程、脂质生物合成过程、甘油磷脂代谢过程、磷脂代谢过程和甘油脂代谢过程等生物过程中,PPAR信号通路中pparα、pparβ、scd1、cpt1和cpt2等多个脂代谢相关基因对军曹鱼幼鱼应对低温胁迫具有关键作用;在糖代谢中,大量基因富集于糖酵解/糖异生、半乳糖代谢、淀粉和蔗糖代谢、戊糖和葡萄糖醛酸酯的相互转化等生物过程,其中g6pc、eno等基因在军曹鱼幼鱼应对低温胁迫时发挥重要调节作用。To investigate the effect of low temperature stress on juvenile cobia(Rachycentron canadum),the fish were reared under normal temperature[(30.5±1.0)℃]and low temperature[(20.0±0.5)℃]for 7 days,followed by genome-based transcriptome sequencing of there liver tissues.Each group included 3 biological replicates.The results showed that a total of approximately 243694134 raw reads were obtained from the 6 sequencing samples,with Q30 scores exceeding 94%for all samples and GC content raging between 47.65%and 48.16%.A total of 4362 differentially expressed genes(DEGs)were identified,including 2793 up-regulated and 1569 down-regulated genes.KEGG pathway enrichment analysis revealed that in lipid metabolism,a significant number of DEGs were enriched in biological processes such as lipid metabolism,lipid biosynthesis,glycerophospholipid metabolism,phospholipid metabolism and glycerideid metabolism.Key lipid metabolism-related genes,including pparα,pparβ,scd1,cpt1,and cpt2 in the PPAR signaling pathway,played crucial roles in the response of juvenile cobia to low temperature stress.In glucose metabolism,numerous genes were enriched in pathways such as glycolysis/gluconeogenesis,galactose metabolism,starch and sucrose metabolism,and pentose and glucuronate interconversions.Among these,genes such as g6pc and eno were found to play important roles in the adaptation of juvenile cobia to low temperature stress.

关 键 词：低温胁迫 军曹鱼 转录组 脂代谢 糖代谢 

分 类 号：Q959.4[生物学—动物学]