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作 者:吴国江 周伟[1] 吕静波 王振国 李岩[1,2] 余忠浩 邓志兰 崔凤娟 李默 徐庆全 周亚星 WU Guojiang;ZHOU Wei;LV Jingbo;WANG Zhenguo;LI Yan;YU Zhonghao;DENG Zhilan;CUI Fengjuan;LI Mo;XV Qingquan;ZHOU Yaxing(Key Laboratory of State Ethnic Affairs Commission of Ecological Agriculture in Horchin Sandy Land,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China;Tongliao Institute of Agricultural and Animal Husbandry Sciences,Tongliao,Inner Mongolia 028000,China)
机构地区:[1]内蒙古民族大学科尔沁沙地生态农业国家民委重点实验室,内蒙古通辽028000 [2]通辽市农牧科学研究所,内蒙古通辽028000
出 处:《干旱地区农业研究》2025年第2期32-43,共12页Agricultural Research in the Arid Areas
基 金:内蒙古自治区自然科学基金(2023LHMS03018);科尔沁沙地生态农业国家民委重点实验室开放基金(MDK2023091);内蒙古自治区科技计划项目(2020GG0102);内蒙古自治区高等学校青年科技英才项目(NJYT22052);2022年度内蒙古自治区直属高校基本科研业务费(GXKY22143)。
摘 要:以不同浓度PEG-6000为渗透剂对30份高粱种质进行模拟干旱胁迫处理,结果显示,15%PEG-6000为高粱种质胁迫适宜浓度。在该处理胁迫浓度下,对279份高粱种质萌发期的耐旱性进行了综合评价。结果表明,9个耐旱指标的变异系数介于21.6%~52.6%之间,并依据各指标的关联度大小确定了发芽势、发芽率和发芽指数3个耐旱关键指标。通过主成分分析,将9个耐旱指标综合成为了3个相互独立的综合指标(主成分值),这3个主成分值的累计贡献率达88.044%。基于主成分分析,利用隶属函数法计算得出高粱种质萌发期耐旱性的综合评价D值,同时基于灰色关联度分析利用DTOPSIS法计算得出高粱种质萌发期耐旱性的相对贴进度,再将D值和相对贴进度求平均,得到新的综合评价值,依据新的综合评价值采用欧式距离及类平均法将279份高粱种质划分为五大类,分别包含29个极耐旱种质、170个耐旱种质、52个中度耐旱种质、12个干旱敏感种质和16个干旱高度敏感种质。In this study,30 sorghum germplasms were treated with simulated drought stress using PEG-6000 at different concentrations as a penetrant.The results showed that 15%PEG-6000 was the appropriate concentration for sorghum germplasm stress.Under this treatment of concentration stress,the drought tolerance of 279 sorghum germplasms was comprehensively evaluated at the germination stage.The results showed that the coefficient of variation of the nine drought tolerance indexes ranged from 21.6%to 52.6%.According to the correlation degree of each index,three key drought tolerance indexes,germination potential,germination rate and germination index were determined.Through principal component analysis,the nine drought tolerance indexes were integrated into three independent comprehensive indexes(principal component values),and the cumulative contribution rate of these three principal component values was 88.044%.Based on principal component analysis,the comprehensive evaluation D value of drought tolerance of sorghum germplasm at germination stage was calculated by membership function method.At the same time,based on grey correlation analysis,the relative sticking progress of drought tolerance of sorghum germplasm at the germination stage was calculated by DTOPSIS method,and then the D value and relative sticking progress were averaged to obtain a new comprehensive evaluation value.Based on the new comprehensive evaluation value,279 sorghum germplasms were classified into five categories using the Euclidean distance and class average method.These categories include 29 extremely drought-tolerant germplasms,170 drought-tolerant germplasms,52 moderately drought-tolerant germplasms,and 28 drought-sensitive germplasms(12 sensitive and 16 highly sensitive).
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