小麦根际促生菌XNT6-1提高小麦抗旱性研究  

Study on enhancing drought resistance in wheat using the rhizosphere growth-promoting bacterium XNT6-1

作　　者：崔天娇 徐虹[1] 李全顺 熊庆祯 刘华伟[1] CUI Tianjiao;XV Hong;LI Quanshun;XIONG Qingzhen;LIU Huawei(College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《干旱地区农业研究》2025年第2期86-94,共9页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金(31772403)。

摘　　要：以前期从小麦‘铜麦6号’根际分离鉴定得到的层出镰孢菌(Fusarium proliferatumon)XNT6-1为试验菌株,以‘铜麦6号’小麦为材料进行种子萌发试验,并通过人工称重控水的盆栽试验设置4个水分处理:正常浇水(no drought,ND)、轻度干旱(low drought,LD)、中度干旱(moderate drought,MD)、重度干旱(severe drought,SD),对干旱胁迫下小麦种子萌发以及幼苗生理生化特性进行分析,探究在干旱胁迫下菌株XNT6-1对小麦种子及幼苗抗旱性的影响。离体平板检测到XNT6-1具有很强的耐旱性,同时具有产铁载体、淀粉酶、蛋白酶和脂酶的能力。在一定干旱条件下,XNT6-1菌体培养液浸种后小麦种子萌发后期叶长及根长分别显著提高了20.4%和17.1%;中度干旱胁迫时,接种XNT6-1使得小麦幼苗鲜质量、干质量、株高和根长分别显著提高16.0%、20.8%、19.5%和23.2%;重度干旱胁迫时,接种XNT6-1使得小麦幼苗鲜质量、干质量、株高和根长分别显著提高36.0%、47.0%、32.4%和37.3%。根际促生菌XNT6-1能够对植物干旱胁迫起到一定缓解作用,是一株抗旱促生优良菌种资源,具有一定的农业应用前景。Fusarium proliferatum XNT6-1,obtained from the rhizosphere isolation and identification of‘Tongmai 6’in the previous stage,was used as the experimental strain.Tongmai 6 was used as the material for the seed germination test,with four water treatments applied through a pot test involving artificial weighing and water control:normal watering(no drought,ND),low drought(LD),moderate drought(MD),and severe drought(SD).The effects of strain XNT6-1 on drought resistance of wheat seeds and seedlings were studied by analyzing seed germination and seedling physiological and biochemical characteristics under drought stress.In vitro,XNT6-1 was found to have a strong drought tolerance and the ability to produce iron carrier,amylase,protease and lipase.Under certain drought conditions,the leaf length and root length of wheat seed at the late stage of germination were significantly increased by 20.4%and 17.1%,respectively,after soaking in XNT6-1 culture medium.Under moderate drought stress,XNT6-1 inoculation significantly increased fresh mass,dry mass,plant height and root length of wheat seedlings by 16.0%,20.8%,19.5%and 23.2%,respectively.Under severe drought stress,XNT6-1 inoculation significantly increased fresh mass,dry mass,plant height and root length of wheat seedlings by 36.0%,47.0%,32.4%and 37.3%,respectively.The rhizosphere growth-promoting bacterium XNT6-1 plays a significant role in alleviating drought stress in plants and is an excellent strain for drought resistance and growth promotion,with promising agricultural applications.

关 键 词：小麦 根际促生菌 抗旱性 促生能力 生理特性 

分 类 号：S182[农业科学—农业基础科学] S512.1[生物学—微生物学] Q939.96