氢气对大鼠缺氧缺血性脑病的抗炎和神经保护作用  

Anti-inflammatory and Neuroprotective Effects of Hydrogen on Hypoxic-ischemic Brain Injury in Rats

作　　者：尚云[1] 王倩楠[1] 申英俊[1] 焦新新 牛海玲 李珍珍 唐成和[1] 崔清洋 SHANG Yun;WANG Qiannan;SHEN Yingjun(Department of Newborn,The First Affiliated Hospital of Xinxiang Medical University,Henan 453100,China)

机构地区：[1]新乡医学院第一附属医院新生儿科,453100

出　　处：《医学研究杂志》2025年第3期80-84,共5页Journal of Medical Research

基　　金：河南省医学科技攻关计划项目(LHGJ20210520);河南省医学教育研究项目(Wjlx2022104)。

摘　　要：目的通过检测脑组织中白细胞介素-6(interleukin 6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α),以及血清中S-100β、神经元特异性烯醇化酶(neuron-specific enolases,NSE)在新生大鼠缺氧缺血性脑病(hypoxic-ischemic encephalopathy,HIE)不同治疗方案中的变化,探讨氢气的抗炎及脑保护作用。方法选取7日龄SD大鼠制作新生大鼠HIE动物模型,按照完全随机数字表法(通过计算机程序采用完全随机数字表法)将其分为假手术组、对照组、实验组1(饱和氢盐水组)、实验组2(亚低温组)、实验组3(联合组)。假手术组不做特殊处理,对照组仅造模,实验组1造模后腹腔注射饱和氢盐水,实验组2造模后行亚低温治疗,实验组3造模后同时进行亚低温联合注射饱和氢盐水治疗。造模后1、3、7天各组均取出10只大鼠留取脑组织,采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测脑组织中IL-6、TNF-α的表达水平,抽血清检测S-100β及NSE的表达水平。结果对照组在造模后1天,脑组织中IL-6、TNF-α以及血清中S-100β及NSE的表达水平明显升高,3天开始回落,7天已降至正常,与假手术组在1、3天比较,差异均有统计学意义(P<0.05);而实验组1和实验组2在造模后1、3天各指标的表达水平均较对照组减低(P<0.05),但高于实验组3及假手术组,差异有统计学意义(P<0.05),实验组1和实验组2各阶段各指标比较,差异无统计学意义(P>0.05)。结论氢气在某种程度上可降低神经系统的炎性反应,同时对新生大鼠HIE有一定的神经保护作用。Objective By detecting the changes of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)in brain tissue,as well as serum S-100βand neuron specific enolases(NSE)in neonatal rats with hypoxic-ischemic encephalopathy(HIE)under different treatment regimens,the anti-inflammatory and neuroprotective effects of hydrogen gas were explored.Methods HIE animal model was made from 7-day-old newborn SD rats,and then divided into sham operation group,control group,test group 1(saturated hydrogen saline group),test group 2(mild hypothermia group),and test group 3(combination group)according to completely random number table method.No special treatment was given in the sham operation group,while the control group only had modeling,test group 1 was intraperitoneally injected with saturated hydrogen saline after modeling,test group 2 was treated with mild hypothermia after modeling,test group3 was treated with mild hypothermia combined with injection of saturated hydrogen saline after modeling.At 1,3 and 7days after modeling,10 rats from each group were decapitated and brain tissue was collected for enzyme-linked immunosorbent assay(ELISA)detection of IL-6 and TNF-α,and serum was collected to detect the expression levels of S-100βand NSE.Results The expression levels of IL-6,TNF-αin the brain tissue,S-100β,and NSE in serum of the control group were increased significantly 1day after modeling,began to decline at 3days,and returned to normal at 7days.Compared with the sham operation group,the expression levels of various indicators in the 1day and 3days were statistically significant(P<0.05).However,the expression levels of various indicators in test group 1and 2decreased compared with the control group on 1day and 3days after modeling(P<0.05),but were higher than those in test group 3 and sham operation group,with statistical significance(P<0.05).There was no statistical significance in the comparison of various indicators between test group 1 and 2 at each stage(P>0.05).Conclusion Hydrogen treatment can reduce the inflammat

关 键 词：缺氧缺血性脑病 氢气 白细胞介素-6 肿瘤坏死因子-α S-100Β 神经元特异性烯醇化酶 大鼠 

分 类 号：R320.34[医药卫生—人体解剖和组织胚胎学]