3所三甲医院儿科门诊空气及物表细菌污染现状与分析  

Current Situation and Analysis of Bacterial Contamination of Air and Surface in Pediatric Outpatient Clinics of Three Tertiary Hospitals

作　　者：王玉娇 付立 刘冰涛 董旭 陈怡 袁静 WANG Yujiao;FU Li;LIU Bingtao(Department of Pediatrics,The Second Affiliated Hospital of Naval Military Medical University,Shanghai 200003,China)

机构地区：[1]海军军医大学第二附属医院儿科,上海200003 [2]海军军医大学第三附属医院消毒供应科,上海200438 [3]海军军医大学第三附属医院儿科,上海200438 [4]海军军医大学第一附属医院感染科,上海200433 [5]海军军医大学第一附属医院儿科,上海200433

出　　处：《医学研究杂志》2025年第3期132-136,共5页Journal of Medical Research

基　　金：军队护理创新与培育专项(2021HL029);海军军医大学护理系“和谐使命-2022”专项课题重点项目(2022HXA02,2022HXA05)。

摘　　要：目的评估3所三甲医院儿科门诊的室内空气及物品表面(以下简称物表)细菌污染状况,为后续提高消毒管理、保护患儿和医护人员提供参考。方法使用大流量生物气溶胶采样器对3所三甲医院儿科门诊不同区域的42份室内空气进行微生物采样监测,物体表面采样法对医护及患者常接触的物表进行采样,并检测细菌菌落形成单位(colony forming units,CFU)。结果B医院儿科门诊的总体空气样本中菌落数显著低于A医院和C医院(P<0.05),A医院的医护及患者常接触物表样本中菌落数显著低于B医院和C医院(P<0.05)。3所医院空气中CFU均超出正常范围,其中A医院候诊大厅病原体显著高于诊室和雾化室(P<0.05);B医院的诊室和候诊大厅区域空气病原体检出差异无统计学意义(P>0.05);C医院候诊大厅和输液室病原体均显著高于诊室(P<0.05)。B医院与C医院中,患者或家属接触的物表检出病原体CFU明显高于医护接触的物表(P<0.05),而A医院患者或家属接触的与医护接触的物表检出病原体CFU差异无统计学意义(P>0.05)。3所医院空气与物表中均可检出致病菌,诊室、候诊大厅、输液室均存在,以金黄色葡萄球菌、蜡样芽孢杆菌和肺炎链球菌为主。结论3所三甲医院儿科门诊均存在不同程度的微生物污染,对常规消毒手段的规范化管理仍需予以重视。Objective To assess bacterial contamination in the indoor air and on item surface(hereinafter referred to as surface)within the pediatric outpatient departments of three tertiary hospitals,and provide reference for improving disinfection management and protecting children and medical staff.Methods A high-flow bioaerosol sampler was used to collected and monitored microorganisms from 42 indoor air samples across various surfaces of pediatric outpatient clinics within three tertiary hospitals.Additionally,surface sampling was employed to examine frequently touched surfaces by both medical staff and patients,with the objective of identifying bacterial colony-forming units(CFU).Results In the pediatric outpatient clinics,the B Hospital showed a significantly lower count of bacterial colonies in general air samples compared to A Hospital and C Hospital(P<0.05).Conversely,A Hospital had a significantly lower count of bacterial colonies on frequently touched surfaces by both medical staff and patients than B Hospital and C Hospital(P<0.05).Airborne CFU exceeded normal levels in all three hospitals.Notably,the waiting hall of A Hospital had a significantly higher concentration of airborne pathogens compared to its consultation and nebulization rooms(P<0.05).At B Hospital,there was no significant difference in airborne pathogen levels between the consultation room and the waiting hall(P>0.05).Meanwhile,the waiting hall and infusion room at C Hospital both had significantly higher pathogen levels than the consultation room(P<0.05).The CFU of pathogens on surfaces frequently contacted by patients or their family members were significantly higher at B Hospital and C Hospital than those contacted by medical staff(P<0.05).In contrast,there was no significant difference in pathogen CFU detected on the surface of patients or family members in hospital A and that of medical staff(P>0.05).Pathogenic bacteria,including staphylococcus aureus,bacillus cereus,and streptococcus pneumoniae,were detected in the air and on surfaces across

关 键 词：儿科门诊 空气微生物 细菌污染 调查研究 消毒 

分 类 号：R134[医药卫生—劳动卫生]