Genomic,transcriptomic,and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin’s orchid  

作　　者：Kai Jiang Birger Lindberg Møller Shaofan Luo Yu Yang David R.Nelson Elizabeth Heather Jakobsen Neilson Joachim Møller Christensen Kai Hua Chao Hu Xinhua Zeng Mohammed Saddik Motawie Tao Wan Guang-Wan Hu Guy Eric Onjalalaina Yijiao Wang Juan Diego Gaitán-Espitia Zhiwen Wang Xiao-Yan Xu Jiamin He Linying Wang Yuanyuan Li Dong-Hui Peng Siren Lan Huiming Zhang Qing-Feng Wang Zhong-Jian Liu Wei-Chang Huang 

机构地区：[1]Shanghai Key Laboratory of Plant Functional Genomics and Resources,CAS Center for Excellence in Molecular Plant Sciences Chenshan Plant Science Research Center,Shanghai Chenshan Botanical Garden,Shanghai 201602,China [2]Eastern China Conservation Centre for Wild Endangered Plant Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602,China [3]Plant Biochemistry Laboratory,Department of Plant and Environmental Science,University of Copenhagen,Copenhagen,Denmark [4]VILLUM Research Center for Plant Plasticity,University of Copenhagen,Copenhagen,Denmark [5]Shanghai Center for Plant Stress Biology,Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 201602,China [6]Department of Microbiology,Immunology and Biochemistry,University of Tennessee Health Science Center,Memphis,TN,USA [7]State Key Laboratory of Plant Diversity and Specialty Crops,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan,China [8]Sino-Africa Joint Research Center,Chinese Academy of Sciences,Wuhan,China [9]University of Antananarivo,Antananarivo,Madagascar [10]The Swire Institute of Marine Science and School of Biological Sciences,The University of Hong Kong,Hong Kong SAR,China [11]PubBio-Tech,Wuhan,China [12]National Key Laboratory of Plant Molecular Genetics,Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China [13]Key Laboratory of Orchid Conservation and Utilization of National Forestry and Grassland Administration at College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou,China [14]Key Laboratory of Plant Design,Chinese Academy of Sciences,Shanghai 200032,China

出　　处：《Molecular Plant》2025年第3期392-415,共24页分子植物(英文版)

基　　金：supported by grants from the Shanghai Landscaping and City Appearance Administrative Bureau(G222406,G232408);Sino-Africa Joint Research Center,CAS(SAJC201607);the Science and Technology Commission of Shanghai Municipality(19390743600);the National Wild Plant Germplasm Resource Centre for Shanghai Chenshan Botanical Garden(ZWGX2102);to W.C.H.and K.J.,and by a Distinguished Investigator grant(NNF190CO05456);from the Novo Nordisk Foundation to B.L.M.E.H.J.N.acknowledges funding from the Novo Nordisk Foundation(0054890);the Independent Research Fund Denmark(1131-00002B and 1051-00083 B).

摘　　要：Angraecum sesquipedale,also known as Darwin’s orchid,possesses an exceptionally long nectar spur.Charles Darwin predicted the orchid to be pollinated by a hawkmoth with a correspondingly long proboscis,later identified as Xanthopan praedicta.In this plant-pollinator interaction,the A.sesquipedale flower emits a complex blend of scent compounds dominated by diurnally regulated oximes(R1R2C=N-OH)to attract crepuscular and nocturnal pollinators.The molecular mechanism of oxime biosynthesis remains unclear in orchids.Here,we present the chromosome-level genome of A.sesquipedale.The haploid genome size is 2.10 Gb and represents 19 pseudochromosomes.Cytochrome P450 encoding genes of the CYP79 family known to be involved in oxime biosynthesis in seed plants are not present in the A.sesquipedale genome nor the genomes of other members of the orchid family.Metabolomic analysis of the A.sesquipedale flower revealed a substantial release of oximes at dusk during the blooming stage.By integrating metabolomic and transcriptomic correlation approaches,flavin-containing monooxygenases(FMOs)encoded by six tandem-repeat genes in the A.sesquipedale genome are identified as catalyzing the formation of oximes present.Further in vitro and in vivo assays confirm the function of FMOs in the oxime biosynthesis.We designate these FMOs as orchid oxime synthases 1-6.The evolutionary aspects related to the CYP79 gene losses and neofunctionalization of FMO-catalyzed biosynthesis of oximes in Darwin’s orchid provide new insights into the convergent evolution of biosynthetic pathways.

关 键 词：Angraecum sesquipedale whole genome sequence white floral scent convergent evolution flavin monooxygenase CYP79 absence 

分 类 号：Q94[生物学—植物学]