DNA damage triggers heritable alterations in DNA methylation patterns in Arabidopsis  

作　　者：Jinchao Li Wenjie Liang Xin-Qiang He Weiqiang Qian 

机构地区：[1]State Key Laboratory of Wheat Improvement,School of Advanced Agricultural Sciences,Peking University,Beijing 100871,China [2]School of Life Sciences,Fudan University,Shanghai 200438,China [3]School of Life Sciences,Peking University,Beijing 100871,China [4]Beijing Life Science Academy,Beijing 102299,China

出　　处：《Molecular Plant》2025年第3期501-512,共12页分子植物(英文版)

基　　金：supported by the National Natural Science Foundation of China(grant no.32270288 to W.Q.;grant no.32400245 to J.L.);the China Postdoctoral Science Foundation(2024M760519 to W.L.);the Beijing Life Science Academy(2024500CA0010 to W.Q.).

摘　　要：It has been hypothesized that DNA damage has the potential to induce DNA hypermethylation,contributing to carcinogenesis in mammals.However,there is no sufficient evidence to support that DNA damage can cause genome-wide DNA hypermethylation.In this study,we demonstrated that DNA single-strand breaks with 3′blocked ends(DNA 3′blocks)not only can reinforce DNA methylation at normally methylated loci but also can induce DNA methylation at normally nonmethylated loci in plants.The CG and CHG hypermethylation tend to localize within gene bodies,with a significant proportion being de novo generated.In contrast,the CHH hypermethylation is concentrated in centromeric and pericentromeric regions,primarily being reinforced methylation.Mechanistically,DNA 3′blocks regulate the DREAM complex to induce CG and CHG methylation.Moreover,they utilize the RdDM pathway to induce CHH hypermethylation.Intriguingly,repair of DNA damage or blocking the DNA damage response can fully abolish CHH hypermethylation and partially rescue CHG hypermethylation but rarely alter CG hypermethylation,indicating that DNA damage-induced symmetric DNA methylation can serve as a form of genetic imprinting.Collectively,these results suggest that DNA damage is an important force driving the emergence and evolution of genomic DNA methylation levels and patterns in plants.

关 键 词：DNA methylation RDDM DREAM DNA damage base excision repair 

分 类 号：Q943.2[生物学—植物学]