反硝化细菌的生长特性及其分离鉴定技术的研究  

Study on the Growth Characteristics and Isolation and Identification of Denitrifying Bacteria

作　　者：房睿 FANG Rui(Xinjiang Uygur Autonomous Region Hydrological Bureau Water Environment Monitoring Center,Urumqi 830000,China)

机构地区：[1]新疆维吾尔自治区水文局水环境监测中心,乌鲁木齐830000

出　　处：《水利科技与经济》2025年第4期126-130,147,共6页Water Conservancy Science and Technology and Economy

摘　　要：硝化和反硝化是水中氮素脱除的两种主要方式。硝化作用将氨氮转化为硝酸盐氮,而反硝化作用则将硝酸盐氮还原为氮气,实现氮素的彻底脱除,两种过程在污水处理和环境保护中具有重要作用。在地下水脱氮领域,反硝化细菌NSA4的发现和应用具有重要的实践意义。研究通过从沉积物中分离并纯化出具有高效反硝化作用的细菌,确保后续研究的针对性和有效性。对NSA4进行形态观察和生理生化检验,可以深入了解该菌种的生物学特性。通过16S rDNA基因序列分析,可以准确鉴定出NSA4的分类学地位和系统发育关系。通过比较NSA4的16S rDNA序列与已知细菌的序列,可以确定NSA4与哪些细菌具有较近的亲缘关系,最终推断出其在自然界中的生态位和功能。Nitrification and denitrification are two main methods of nitrogen removal from water.Nitrification converts ammonia nitrogen to nitrate nitrogen,while denitrification reduces nitrate nitrogen to nitrogen gas for complete nitrogen removal.Both of these processes are of great significance in wastewater treatment and environmental protection.In the field of groundwater denitrification,the discovery and application of denitrifying bacteria NSA4 are of great practical significance.By isolating and purifying highly denitrification bacteria from sediment,this study ensured that subsequent studies were targeted and effective.The biological characteristics of NSA4 can be deeply understood by morphological observation and physiological and biochemical examination.Finally,the phylogenetic relationship of NSA4 can be accurately identified by 16S rDNA gene sequence analysis.By comparing the 16S rDNA sequence of NSA4 with that of known bacteria,we can determine which bacteria NSA4 is closely related to and finally infer its niche and function in nature.

关 键 词：氮素脱除 分离鉴定 硝酸盐污染 地下水处理 

分 类 号：X523[环境科学与工程—环境工程]