非洲猪瘟病毒R298L基因缺失毒株的构建及其致病性与免疫原性评价  

Construction and evaluation of pathogenicity and immunogenicity of African swine fever virus R298L gene deletion strain

作　　者：范许许 李伟伟 朱兆宇 裴丹诗 王一卓 何路 李熙忠 任青峰 郑海学[1] 朱紫祥[1] FAN Xuxu;LI Weiwei;ZHU Zhaoyu;PEI Danshi;WANG Yizhuo;HE Lu;LI Xizhong;REN Qingfeng;ZHENG Haixue;ZHU Zixiang(State Key Laboratory for Animal Disease Control and Prevention/Colle ge of Veterinary Medicine of Lanzhou University/Lanzhou Veterinary Research Institute,Chinese Academy of A gricultural Sciences,Lanzhou 730000,China)

机构地区：[1]中国农业科学院、兰州兽医研究所、兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室,甘肃兰州730000

出　　处：《中国兽医科学》2025年第3期285-290,共6页Chinese Veterinary Science

基　　金：“十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目(2024KJ14);国家重点研发计划项目(2021YFD1800300);中央高校基本科研业务费专项(lzujbky-2022-ct02);甘肃省科技重大专项(22ZD6NA001,22ZD6NA012);国家自然科学基金青年项目(32202781,32302857);甘肃省青年科技基金计划项目(22JR5RA034);中国博士后科学基金项目(2023M743830);国家生猪产业技术体系项目(CARS-35,CARS-39-13);国家生猪技术创新中心先导科技项目(NCTIP-XD/C03);中国农业科学院创新计划项目(CAAS-CSLPDCP-2023002,CAAS-ASTIP-2024-LVRI);甘肃省创新群体项目(23JRRA546)。

摘　　要：为了探究非洲猪瘟病毒(ASFV)R298L基因的功能,解析ASFV的致病机制,为基因缺失减毒疫苗的研发提供候选毒株,首先利用同源重组技术构建R298L基因缺失的非洲猪瘟病毒株ASFV-ΔR298L,随后通过动物实验在猪体内比较ASFV-ΔR298L与亲本毒株ASFV CN/GS/2018致病性和免疫应答水平的差异。结果表明,感染亲本毒株的动物发热严重,在感染后13 d内全部死亡,解剖学观察显示多器官发生严重的病变,而感染ASFV-ΔR298L重组病毒的动物发热水平显著下降,在观察期内全部存活,组织器官未发生病变,并且在后期产生较高水平的中和抗体。以上数据为解析R298L病毒蛋白的功能和阐明非洲猪瘟病毒的致病机制提供了理论依据,为基因缺失候选疫苗的研制提供了参考。To explore the function of African swine fever virus(ASFV)R298L gene,elucidate the pathogenic mechanism of ASFV,and provide a candidate strain for the vaccine research,the gene deletion ASFV-△R298L was constructed by homologous recombination.The pathogenicity and immune response of ASFV-△R298L and ASFV CN/GS/2018 were evaluated in pigs.The results showed that animals infected with ASFV-WT had severe fever and all died within 13 days after infection,which severe lesions occurred in multiple organs,but the animals infected with ASFV-△R298L had no fever or lesions in organs and all survived during the observation period.More importantly,pigs infected with ASFV-△R298L produced high levels of neutralizing antibody in late stage.In summary,these findings provide insights into analysis the pathogenic mechanism of ASFV,and provide important ideas for the development of gene deletion candidate vaccines.

关 键 词：非洲猪瘟病毒 R298L基因 缺失毒株 致病性 免疫保护 

分 类 号：S852.659.1[农业科学—基础兽医学]