槲皮素缓减T-2毒素对细胞中猪轮状病毒复制的影响  

Quercetin attenuates the effect of T-2 toxin on porcine rotavirus replication in cells

作　　者：孙彤 高美晨[2] 林洪金 SUN Tong;GAO Meichen;LIN Hongjin(Nanjing Agricultural University,Nanjing 210000,China;Northeast A gricultural University,Harbin 150030,China)

机构地区：[1]南京农业大学,江苏南京210000 [2]东北农业大学,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2025年第3期403-414,共12页Chinese Veterinary Science

基　　金：黑龙江省自然科学基金项目(LH2023C028)。

摘　　要：为了探究槲皮素和/或T-2毒素在体外对猪轮状病毒(PoRV)复制的影响,检测PoRV在非洲绿猴肾细胞(MA104细胞)内的复制水平以及添加槲皮素和/或T-2毒素后对细胞氧化应激、自噬水平和PI3K/AKT通路表达指标的影响。结果表明,添加T-2毒素可提高PoRV在细胞内复制水平;染色结果显示,添加槲皮素后PoRV+Que组与对照组相比,细胞内氧化应激和自噬水平显著上升,但与T-2+PoRV组和T-2+PoRV+Que组相比,氧化应激和自噬水平均有所缓解,病毒复制水平显著降低;PoRV、自噬相关基因和通路的mRNA和蛋白表达水平的检测结果表明,T-2毒素处理后,细胞氧化应激水平、自噬水平、病毒复制水平均上升;经槲皮素处理后,细胞内氧化应激水平、自噬基因表达水平、病毒复制水平相对下降。结论:槲皮素能缓减T-2毒素对细胞内病毒复制、氧化应激和自噬水平的影响。In order to investigate the effects of quercetin and/or T-2 toxin on porcine rotavirus(Po RV) replication in vitro,Po RV replication levels in African green monkey kidney cells(MA104 cells)and the effects of quercetin and/or T-2 toxin addition on cellular oxidative stress,autophagy levels,and PI3K/AKT pathway expression indicators were examined.The results showed that adding T-2 toxin could improve the replication level of Po RV in cells.The staining results showed that the intracellular oxidative stress and autophagy levels in the Po RV+Que group increased significantly compared with the control group after quercetin addition,but the levels of oxidative stress and autophagy were alleviated compared with the T-2+PoRV and T-2+PoRV+Que groups,and the viral replication level was significantly decreased.The m RNA and protein expression levels of Po RV and autophagy related genes and pathways were detected.The results showed that the oxidative stress level,autophagy level and viral replication level were increased after T-2 toxin treatment.After quercetin treatment,the levels of oxidative stress,autophagy gene expression and viral replication decreased.In conclusion,quercetin can reduce the effects of T-2 toxin on the level of intracellular viral replication,oxidative stress and autophagy.

关 键 词：T-2毒素 猪轮状病毒 槲皮素 氧化应激 自噬 

分 类 号：S852.4[农业科学—基础兽医学]