Mass Spectrometry Imaging for Cellular-level Analysis:Advancesand Applications on Medical Research  

作　　者：YU Yile FAN Jinghan LIU Huihui NIE Zongxiu 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistryfor Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,P.R.China [2]University of Chinese Academy of Sciences,Beijing 100049,P.R.China

出　　处：《Chemical Research in Chinese Universities》2025年第2期254-265,共12页高等学校化学研究(英文版)

基　　金：supported by the National Natural Science Foundation of China(Nos.22334007,22274160,22304175).

摘　　要：Mass spectrometry(Ms)is widely used in medical applications,such as pharmacokinetics,drug discovery,and clinical diagnostics,as it enables medical researchers and practitioners to gain insights into pathogenic mechanisms,identify biomarkers for diagnosis and monitoring,and develop new treatments.Mass spectrometry imaging(Msi)is a powerful analytical technique that combines the spatial information from imaging with the chemical information from MS.Ms assists medical researchers in various ways,including biomarker discovery,drug development and evaluation,personalized medicine,tissue imaging,and histochemical analysis.In addition,Msi not only provides high-resolution images of sample structures and enables researchers to identify specific cell types and their functions,but also enables the simultaneous visualization of multiple biomolecules within a single cell and allows researchers to study complex biological processes with greater precision.In this review,we summarize recent advances in Msi for single cells and cellular-level analysis and discuss how this technology can be used to improve our understanding of diseases and develop new treatments.The potential challenges and limitations of Msl in single-cell analysis,as well as prospects in this field,are also highlighted.

关 键 词：Single cell analysis Cellular-level Matrix-assisted laser desorption ionization mass spectrometry imaging(MALDI-MSI) Secondary ion mass spectrometry(SIMS)-lmaging Desorption electrospray ionization mass spectrometry imaging(DES-MSl) 

分 类 号：O657.63[理学—分析化学]