ZmDIR11调控玉米抗盐胁迫机制研究  

Mechanism of ZmDIR11 regulating resistance to salt stress in maize

作　　者：赵志雄 关媛[2] 王慧[2] 顾炜 于典司[2] 郑洪建[2] 秦涛 ZHAO Zhixiong;GUAN Yuan;WANG Hui;GU Wei;YU Diansi;ZHENG Hongjian;QIN Tao(College of Fisheries and Life Sciences,Shanghai University of Ocean,Shanghai 201306,China;Institute of Crop Breeding and Cultivation,Shanghai Academy of Agricultural Sciences/CIMMYT-China Special Maize Research Center/Shanghai Engineering Research Center of Specialty Maize/Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201403,China)

机构地区：[1]上海海洋大学水产与生命科学学院,上海201306 [2]上海市农业科学院作物育种栽培研究所/CIMMYT-中国特用玉米研究中心/上海特用玉米工程技术研究中心/上海市农业遗传育种重点实验室,上海201403

出　　处：《华中农业大学学报》2025年第2期158-169,共12页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(32101754,32001555,32172033);上海市农业科技创新项目[沪农科(K2024004)]。

摘　　要：为挖掘玉米抗盐代谢胁迫调控关键基因,克隆玉米DIR基因家族成员ZmDIR11(Zm00001d012432),对其基因及蛋白结构进行预测,分析表达模式,并进一步在玉米B73-EMS突变体中探索ZmDIR11参与耐盐胁迫的调控模式。结果显示,ZmDIR11编码包含Dirigent结构域的多肽,其启动子区域ARE、MYB、MYC、STRE、LTR、MBS结构元件均与非生物胁迫调控相关。ZmDIR11在玉米根系中优势表达,且受盐胁迫诱导上调表达,亚细胞定位于内质网中。在盐胁迫处理后突变体玉米植株生长势及物质积累显著低于野生型B73材料,且氧化代谢调控路径、脯氨酸积累、叶绿素合成、木脂素积累、Na+/K+转运通路被抑制,相关代谢指标显著低于野生型B73材料。结果表明,ZmDIR11在玉米中正向调控玉米抗盐代谢通路,突变后降低了玉米的耐盐性。The ZmDIR11 gene,a member of the DIR gene family,was cloned from maize and its sequence of gene and structures of protein were predicted to mine key genes involved in regulating the resistance to salt stress in maize.The patterns of gene expression were analyzed to further investigate the regulatory mode of ZmDIR11 in salt stress tolerance in maize B73-EMS mutants.The results showed that ZmDIR11 encoded a peptide containing the Dirigent domain,and its promoter region contained structural elements including ARE,MYB,MYC,STRE,LTR,and MBS,all associated with the regulation of abiotic stress.ZmDIR11 was predominantly expressed in maize roots and upregulated under salt stress,with subcellular localization in the endoplasmic reticulum.It was found that the growth and material accumulation of mutant maize plants after salt stress treatment were significantly lower than those of wild-type B73,and the regulation pathway of oxidative metabolism,the accumulation of proline,the synthesis of chlorophyll,the accumulation of lignin,and the transport pathway of Na+/K+ were inhibited.Relevant metabolic indexes of mutant maize plants were significantly lower than those of wild-type B73.It is indicated that ZmDIR11 positively regulates the metabolic pathway of salt tolerance in maize,and its mutation reduces the salt tolerance in maize.

关 键 词：玉米 生物信息学 ZmDIR11 DIRs基因 盐胁迫 耐盐机制 

分 类 号：S513.3[农业科学—作物学]