四川水稻主栽品种抗胡麻叶斑病评价及抗病生理生化机制研究  

Evaluation of resistance to sesame leaf spot disease and physiological and biochemical mechanisms of disease resistance in main rice varieties in Sichuan Province

作　　者：李文韬 贾燕 罗秀梅 彭安春 吴春先 王学贵[1] LI Wentao;JIA Yan;LUO Xiumei;PENG Anchun;WU Chunxian;WANG Xuegui(College of Agriculture,Sichuan Agricultural University,Chengdu 611130,China;Rongxian Planting Industry Service Center,Zigong 643100,China;Plant Protection Station of Department of Agriculture and Rural Affairs,Sichuan Province,Chengdu 610041,China)

机构地区：[1]四川农业大学农学院,成都611130 [2]四川省荣县种植业服务中心,自贡643100 [3]四川省农业农村厅植物保护站,成都610041

出　　处：《华中农业大学学报》2025年第2期190-199,共10页Journal of Huazhong Agricultural University

基　　金：国家重点研发计划项目(2018YFD0200300)。

摘　　要：为明确目前四川水稻主栽品种对胡麻叶斑病的抗病性及生理生化抗性机制,采用盆栽抗病性鉴定方法评价了20种四川水稻主栽水稻品种和5种对照品种对胡麻叶斑病的抗性水平,测定了不同抗性水平品种叶片的5种防御酶活性,利用气相色谱/质谱(GC/MS)分析了抗病和感病品种中水稻幼苗叶片的次生代谢物质。结果显示,渝香糯1号和浙粳优4号为高抗品种,秀水134、浙禾622、浙禾香2号、浙粳优77和浙粳优1578等5个品种为中抗品种,其余品种均为感病品种;抗病水稻品种和感病水稻品种中超氧化物歧化酶(SOD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和过氧化氢酶(CAT)等5种酶活性均先升高后降低,抗病品种的酶活性显著高于感病品种;总共得到18种次生代谢物,其中核糖醇、D-葡萄糖、苹果酸、2,3,4-三羟基丁酸等4种代谢物在水稻抗病品种和感病品种以及同种品种接菌与不接菌处理的含量均差异显著。因此,水稻抗病品种可通过提升5种防御酶的活性与4种次生代谢物的含量来抵御胡麻叶斑病的侵染。The resistance to sesame leaf spot disease of 20 main rice varieties and 5 control varieties in Sichuan Province was evaluated with potted plant disease resistance identification method to study the resistance to sesame leaf spot disease and the physiological and biochemical mechanisms of disease resistance in the main rice varieties in Sichuan Province.The activity of 5 defense enzymes was determined in the leaves of different resistant varieties.The secondary metabolites in the leaves of rice seedlings in resistant and susceptible varieties were analyzed with gas chromatography/mass spectrometry(GC/MS).The results showed that Yuxiangnuo 1 and Zhejingyou 4 were highly resistant varieties,while 5 varieties including Xiushui 134,Zhehe 622,Zhehexiang No.2,Zhejingyou 77 and Zhejingyou 1578 had moderate resistance,and the rest were susceptible varieties.The activity of 5 enzymes including superoxide dismutase(SOD),polyphenol oxidase(PPO),phenylalanine ammonia lyase(PAL),peroxidase(POD),and catalase(CAT) increased first and then decreased in both resistant and susceptible rice varieties,but the activity of enzyme in resistant varieties was significantly higher than that in susceptible varieties.A total of 18 secondary metabolites were obtained,among which 4 metabolites including ribosyl alcohol,D-glucose,malic acid,and 2,3,4-trihydroxybutyric acid had significant differences in content between resistant and susceptible rice varieties,and between inoculated and non-inoculated treatment of the same variety.It is indicated that rice resistant varieties can resist the invasion of sesame leaf spot disease by increasing the activity of 5 defense enzymes and the content of four secondary metabolites.

关 键 词：水稻 胡麻叶斑病 抗病性 次生物质 气相色谱/质谱(GC/MS) 

分 类 号：S511[农业科学—作物学] Q945.8[农业科学—植物病理学]