基于全基因组测序的多粘类芽孢杆菌NBmelon-1生防机制探究及验证  

Exploration and verification of biocontrol mechanisms of Paenibacillus polymyxa NBmelon-1 based on whole genome sequencing and analysis

作　　者：董文杰 郝芳敏 臧全宇 马二磊 丁伟红 王毓洪 DONG Wenjie;HAO Fangmin;ZANG Quanyu;MA Erlei;DING Weihong;WANG Yuhong(Ningbo Academy of Agricultural Sciences/Ningbo Key Laboratory for Quality Control and Resistance Breeding of Characteristic Horticultural Crops,Ningbo 315040,China;Zhejiang Wanli University,Ningbo 315199,China)

机构地区：[1]宁波市农业科学研究院/宁波市特色园艺作物品质调控和抗性育种重点实验室,宁波315040 [2]浙江万里学院,宁波315199

出　　处：《华中农业大学学报》2025年第2期200-211,共12页Journal of Huazhong Agricultural University

基　　金：宁波市科技局重点研发计划暨“揭榜挂帅”项目(2023Z115);浙江省西甜瓜良种育繁推科技创新平台项目(ZJ2019-80);国家西甜瓜产业技术体系建设专项(CARS-25)。

摘　　要：为研究多粘类芽孢杆菌NBmelon-1的抑菌促生机制,采用三代PacBio测序技术进行全基因组测序,分析潜在的功能基因以及预测次级代谢产物合成基因簇等;检测抑菌促生相关基因的表达情况;利用粗提物拮抗试验明确抑菌物质的主要成分,并验证该菌促生潜力,进一步明确其生防机制。结果显示,多粘类芽孢杆菌NBmelon-1基因组大小为5.7 Mb,GC含量为45.68%,含有4 984个编码基因;基因组中存在大量与诱导植物抗病性、水解酶和促进植物生长相关的基因,共预测到5个脂肽类次级代谢产物合成基因簇;采用酸沉淀法从NBmelon-1培养物中分离获得的粗提物对多种甜瓜病原真菌具有明显的抑菌活性,说明其中含有抑菌物质。促生潜力试验表明该菌株能够合成生长激素IAA、具有产铁载体能力,并为植物提供氮元素。以上结果表明,菌株NBmelon-1是一株多功能生防菌,具有广阔的开发应用前景。Paenibacillus polymyxa NBmelon-1 is an efficient biocontrol bacterium isolated from the roots of sweet melons.The third-generation of PacBio sequencing technique was used to sequence the whole genome of strain NBmelon-1 to study the biocontrol mechanisms of P.polymyxa NBmelon-1.The potential functional genes were analyzed and clusters of genes involved in the synthesis of secondary metabolites were predicted.The expression of genes related with antibacterial effect and promoting the growth of plants was detected.The antagonism test of crude extracts was used to identify the main components of antibacterial substances and validate the potential of the bacteria to promote the growth of plants,further clarifying its biocontrol mechanism.The results showed that the genome of P.polymyxa NBmelon-1 was 5.7Mb,with a GC content of 45.68% and 4 984 coding genes.There were a large number of genes involved in inducing plant disease resistance,hydrolytic enzymes,and promoting plant the growth of plants in the genome.A total of five clusters of genes involved in the synthesis of secondary metabolites of lipopeptides were predicted.The crude extracts isolated from NBmelon-1 culture with acid precipitation method had significant antibacterial activity against a variety of pathogenic fungi from melon,indicating the presence of antibacterial substances.The results of growth potential test showed that the strain was capable of synthesizing growth hormone IAA,producing iron carriers,and providing nitrogen elements for plants.It is indicated that P.polymyxa NBmelon-1 is a multifunctional biocontrol bacterium with broad prospects for development and application in agricultural production.

关 键 词：多粘类芽孢杆菌 全基因组 次级代谢产物 抑菌作用 

分 类 号：Q933[生物学—微生物学] S476[农业科学—农业昆虫与害虫防治]