植物非组培遗传转化体系及其应用  

Genetic transformation system of plants with non-tissue culture and its application

作　　者：冯梦琦 王若雨 司马璐 姜楠 翟晓巧[3] 赵振利[1] 范国强[1] FENG Mengqi;WANG Ruoyu;SIMA Lu;JIANG Nan;ZHAI Xiaoqiao;ZHAO Zhenli;FAN Guoqiang(College of Forestry,Hennan Agricultural University,Zhengzhou 450046,China;Institute of Non-timber Forestry,Chinese Academy of Forestry Sciences,Zhengzhou 450003,China;Henan Academy of Forestry Sciences,Zhengzhou 450002,China)

机构地区：[1]河南农业大学林学院,郑州450046 [2]中国林业科学研究院经济林研究所,郑州450003 [3]河南省林业科学研究院,郑州450002

出　　处：《华中农业大学学报》2025年第2期228-242,共15页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(32401613);中国博士后科学基金项目(2024M750814)。

摘　　要：植物高效稳定的遗传转化体系是基因功能解析和分子育种的重要技术支撑,然而组织培养体系不完善严重阻碍其在诸多植物尤其作物中的应用。为了解决上述问题,近年来建立了不少高效的非组培遗传转化体系。本文着重综述农杆菌介导的非组培遗传转化体系,包括花器官侵染法、切-浸-芽(cut-dip-budding,CDB)递送法、依赖再生活力的活体注射递送法(regenerative activity-dependent in planta injection delivery,RAPID)、种子侵染法、发育调控基因(development regulatory,DR)辅助转化法和病毒递送法在植物中的应用现状、转化效率影响因素和基因编辑应用;总结了不依赖农杆菌的花粉管通道法、粒子轰击法和纳米递送法在植物中的应用,旨在为更多物种建立高效、简便、基因型不依赖的非组培遗传转化体系提供参考,助力植物功能基因研究和分子育种实践。The efficient and stable genetic transformation system of plants is an important technical support for the analysis of gene function and molecular breeding.However,the imperfect tissue culture system seriously hinders its application in many plants,especially in crops.In recent years,many efficient genetic transformation systems of plants with non-tissue culture have been established to solve the problems mentioned above.This article focuses on the genetic transformation system of plants with non-tissue culture mediated by Agrobacterium tumefaciens in terms of the current status of application,factors affecting transformation efficiency,and applications of gene editing with flower organ infection,cut-dip-budding(CDB) delivery,regenerative activity dependent in plant injection delivery(RAPID),seed inoculation,development regulatory(DR) assisted transformation,and virus delivery in plants.The application of pollen tube channel method,particle bombardment method and nano delivery method in plants independent of Agrobacterium tumefaciens was summarized.It will provide reference for establishing efficient,simple and genotype-independent genetic transformation system with non-tissue culture in more species and assisting the studies on plant functional genes and the practices of molecular breeding.

关 键 词：植物遗传转化 非组培 农杆菌 基因编辑 基因型不依赖 

分 类 号：S-1[农业科学]