原子吸收分光光度法测定复方氨基酸注射液(18AA-I)中钠  

Determination of sodium content in Compound Amino Acid Injection(18AA-I)by atomic absorption spectrophotometry

作　　者：张俊朋 连莹 王宣宣 ZHANG Junpeng;LIAN Ying;WANG Xuanxuan(Henan Institute for Drug and Medical Device Inspection(Henan Vaccine Issuance Center),Zhengzhou Key Laboratory for Quality Research and Evaluation of Generic Drug,Zhengzhou 450018,China)

机构地区：[1]河南省药品医疗器械检验院(河南省疫苗批签中心),郑州市仿制药质量研究与评价重点实验室,郑州450018

出　　处：《化学分析计量》2025年第3期79-85,共7页Chemical Analysis And Meterage

基　　金：2023年国家药品标准制修订研究课题(2023H044)。

摘　　要：建立原子吸收分光光度法测定复方氨基酸注射液(18AA-I)中钠含量。样品以0.5%硫酸铜的硝酸溶液为溶剂,乙炔空气燃助比为1.3∶10,检测波长为589 nm,狭缝宽度为0.2 nm,以标准曲线法定量。钠的质量浓度在1~5μg/mL范围内与吸光度值线性关系良好,相关系数为0.9994,方法检出限为0.028μg/mL。样品测定结果的相对标准偏差为0.6%(n=6),空白基质加标回收率为98.4%~101.9%。该方法准确简便,能够有效消除样品中基质干扰,适用于复方氨基酸注射液(18AA-I)中钠含量的测定。An atomic absorption spectrophotometric method was established for the determination of sodium content in Compound Amino Acid Injection(18AA-I).The sample was dissolved in a nitric acid solution containing 0.5%copper sulfate.The ratio of acetylene to air was 1.3∶10.The detection wavelength was set at 589 nm,and the slit width was 0.2 nm.The standard curve method was adopted for quantification.The mass concentration of sodium showed a good linear relationship with the absorbance value within the range of 1-5μg/mL,with the correlation coefficient of 0.9994.The detection limit of the method was 0.028μg/mL.The relative standard deviation of the sample determination results was 0.6%(n=6),and the spiked recoveries rate of the blank matrix were 98.4%-101.9%.This method is accurate and simple,and it can effectively eliminate the matrix interference in the sample.It is suitable for the determination of sodium content in Compound Amino Acid Injection(18AA-I).

关 键 词：复方氨基酸注射液(18AA-I) 原子吸收分光光度法 钠含量 

分 类 号：O657.32[理学—分析化学]