抑肽酶对防治梨小食心虫的两种植物源农药的增效作用  

Synergistic effect of aprotinin with two botanical pesticides against oriental fruit moth Grapholita molesta

作　　者：李俊杰 杨晓燕 唐慧琳 高俊恒 郭子坤 郭春阳 王冬寒 叶佳成 袁向群[1] 李怡萍[1] Li Junjie;Yang Xiaoyan;Tang Huilin;Gao Junheng;Guo Zikun;Guo Chunyang;Wang Donghan;Ye Jiacheng;Yuan Xiangqun;Li Yiping(Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education,Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi Province,China)

机构地区：[1]西北农林科技大学植物保护学院,农业农村部西北黄土高原作物有害生物综合治理重点实验室,植保资源与病虫害治理教育部重点实验室,陕西杨凌712100

出　　处：《植物保护学报》2025年第1期105-112,共8页Journal of Plant Protection

基　　金：国家自然科学基金(32172409,31871971);陕西省科技重大专项(2020zdzx03-03-02)。

摘　　要：为提高植物源药剂对梨小食心虫Grapholita molesta的防治效果,测定饲喂含抑肽酶的人工饲料后梨小食心虫2龄幼虫中肠内总蛋白酶、胰蛋白酶和胰凝乳蛋白酶的活性,采用饲料混毒法测定0.20 mg/L抑肽酶对1%印楝素水分散粒剂和0.3%苦参碱水剂的增效作用。结果表明:饲喂含0.20 mg/L抑肽酶的人工饲料12 h后,梨小食心虫2龄幼虫中肠内总蛋白酶、胰蛋白酶和胰凝乳蛋白酶的活性均与对照无显著差异;饲喂36 h后,这3种蛋白酶的活性均较对照显著下降。当0.20 mg/L抑肽酶分别与LC_(10)、LC_(30)、LC_(50)的1%印楝素水分散粒剂混配饲喂后,梨小食心虫2龄幼虫的实际死亡率分别为29.16%、43.06%和66.67%,均显著高于预测死亡率;当0.20 mg/L抑肽酶分别与LC_(10)、LC_(30)和LC_(50)的0.3%苦参碱水剂混配饲喂后,梨小食心虫2龄幼虫的实际死亡率分别为44.44%、58.33%和68.89%,均显著高于预测死亡率。表明抑肽酶通过抑制梨小食心虫中肠蛋白酶活性对印楝素或苦参碱产生增效作用。To improve the control effect of botanical pesticides on oriental fruit moth Grapholita mo‐lesta,the activities of total protease,trypsin and chymotrypsin in the midgut of the second larvae of G.molesta were measured after feeding an artificial diet containing 0.20 mg/L aprotinin.The synergistic ef‐fect of 0.20 mg/L aprotinin on 1%azadirachtin WG and 0.3%AS was determined by feeding on the diet containing these toxic substances.The results showed that after feeding the artificial diet containing aprotinin for 12 h,the activities of total protease,trypsin and chymotrypsin in the midgut of the second instar larvae were not significantly different from the control group.After feeding for 36 h,the activities of these three proteases decreased significantly compared with the control group.When 0.20 mg/L apro‐tinin was mixed with LC_(10),LC_(30) and LC_(50) of 1%azadirachtin WG,the actual mortality rates were 29.16%, 43.06% and 66.67%, respectively, which were significantly higher than the predicted mortality.When 0.20 mg/L aprotinin was mixed with LC_(10), LC_(30) and LC_(50) of 0.3% matrine AS, the actual mortalityrates were 44.44%, 58.33% and 68.89%, respectively, which were significantly higher than the pre‐dicted mortality. These results indicate that aprotinin has a synergistic effect on azadirachtin or matrineby inhibiting the activity of the midgut proteases in G. molesta.

关 键 词：梨小食心虫 抑肽酶 苦参碱 印楝素 增效作用 

分 类 号：S433.39[农业科学—农业昆虫与害虫防治]