我国黄淮海地区大豆症青相关病毒的鉴定及其抗(耐)性材料筛选  

Identification of soybean stay-greenassociated virus in the Huang-Huai-Hai region of China and screening of disease-resistant soybean materials

作　　者：程信歌 肖燕敏 濮若思 张子龙 屈浩然 丁嘉瑜 申宇航 李凯 Cheng Xinge;Xiao Yanmin;Pu Ruosi;Zhang Zilong;Qu Haoran;Ding Jiayu;Shen Yuhang;Li Kai(State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,National Innovation Platform for Soybean Breeding and Industry-Education Integration,Zhongshan Biological Breeding Laboratory,Key Laboratory of Biology and Genetics Improvement of Soybean,Ministry of Agriculture and Rural Affairs,College of Agriculture,Nanjing Agricultural University,Nanjing 210095,Jiangsu Province,China)

机构地区：[1]南京农业大学农学院,农业农村部大豆生物学与遗传育种重点实验室,生物育种钟山实验室,国家大豆生物育种产教融合创新平台,作物遗传与种质创新利用全国实验室,南京210095

出　　处：《植物保护学报》2025年第1期123-135,共13页Journal of Plant Protection

基　　金：财政部和农业农村部国家现代农业产业技术体系(CARS-004);生物育种钟山实验室课题(ZSBBL-KY2023-03);江苏省现代作物生产协同创新项目(JCIC-MCP);现代作物生产省部共建协同创新中心项目(CIC-MCP)。

摘　　要：为探究黄淮海地区大豆症青连年暴发的原因并筛选优异的抗(耐)性大豆新品种(系),在黄淮海地区不同地区采集疑似病样209份,利用特异性引物对病样的叶片和豆荚果皮进行大豆症青相关病毒(soybean stay-green associated virus,SoSGV)的检测,随机选取21个阳性样品进行全基因组测序及系统发育分析,构建SoSGV侵染性克隆进行侵染性试验,并以普通棕色叶蝉Orosius orientalis为SoSGV传毒介体对274份我国新育成大豆品种(系)进行抗性评价。结果显示:采集自黄淮海各省份表现症青的大豆病样中SoSGV的检出率均超过70%,甚至在个别省份检出率达100%,初步推断SoSGV是我国黄淮海地区大豆症青的主要病原,且该病害有向西北大豆产区扩散的趋势;21条阳性样品的SoSGV全基因组与已知的6条SoSGV全基因组间的核苷酸序列同源性均超过92%,系统发育分析显示该病毒共有3个进化群体,以群体Ⅰ为主要流行种群;与其他双生病毒的系统发育分析显示SoSGV属于一种新型重组双生病毒;构建的SoSGV侵染性克隆可成功侵染本氏烟Nicotiana benthamiana;在274份大豆新品种(系)中有271份对SoSGV表现为易感,且出现典型症青症状,仅有3份为耐病新品种(系)。To investigate the causes of recurrent outbreaks of soybean stay-green syndrome in the Huang-Huai-Hai region and to screen soybean cultivars(lines)with resistance(or tolerance)to this dis‐ease,a total of 209 suspected samples were collected from different provinces within the region.Specif‐ic primers were used to detect soybean stay-green associated virus(SoSGV)in the leaves and pod peri‐carp tissues of the collected samples.Whole-genome sequencing and phylogenetic analysis were con‐ducted on 21 randomly selected SoSGV-positive samples,and infectious clones of SoSGV were con‐structed for infectivity assays.Additionally,274 newly developed soybean cultivars(lines)in China were evaluated for resistance to SoSGV using the common brown leafhopper Orosius orientalis as thetransmission vector. The results revealed that SoSGV detection rates exceeded 70% in samples collectedacross provinces in the Huang-Huai-Hai region, with some provinces reaching 100%. This result sug‐gested that SoSGV was the primary pathogen causing soybean stay-green syndrome in China, with atrend of spread toward northwestern soybean-producing areas. The genome nucleotide sequences of27 SoSGV isolates shared over 92% identity. Phylogenetic analysis identified three evolutionary groups,with group I as the predominant epidemic population. Comparative phylogenetic analysis with othergeminiviruses revealed that SoSGV was a novel recombinant geminivirus. Infectious clones of SoSGVsuccessfully infected Nicotiana benthamiana. Among the 274 soybean cultivars (lines) evaluated, 271were highly susceptible to SoSGV and exhibited typical stay-green symptoms, while only three culti‐vars (lines) showed resistance to this disease.

关 键 词：大豆 症青 大豆症青相关病毒 普通棕色叶蝉 抗性鉴定 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]