羟丙基甲基纤维素制剂颗粒粒径大小对其杀螨活性的影响  

Effect of particle size of hydroxypropyl methylcellulose on acaricidal activity

作　　者：王琳琳 李健生 李卫国 梁殷泽 崔建国 黄燕敏 WANGLinlin;LI Jiansheng;LI Weiguo;LIANG Yinze;CUI Jianguo;HUANG Yanmin(School of Chemistry and Materials Science,Nanning Normal University,Nanning 530001,China;Guangxi Tianyuan Biochemical Co.,Ltd.,Nanning 530001,China)

机构地区：[1]南宁师范大学化学与材料科学学院,南宁530001 [2]广西田园生化股份有限公司,南宁530001

出　　处：《农药》2025年第4期250-254,259,共6页Agrochemicals

基　　金：物理环保物理作用机制(窒息)害螨灭杀剂的研究和开发(AB21220015)。

摘　　要：[目的]研究羟丙基甲基纤维素纳米制剂的粒径大小对柑橘全爪螨杀螨活性的影响。[方法]以羟丙基甲基纤维素为原料,通过高速搅拌切割和超声分散方法,分别制备了3种粒径范围在185~200、330~380、710~780 nm区间的纳米颗粒制剂,同时采用激光共聚焦显微镜、金相显微镜和电子扫描显微镜对柑橘全爪螨的死亡状态进行生物作用机制分析。[结果]3种纳米制剂均对柑橘全爪螨表现出明显杀螨活性,尤以粒径在330~380 nm的制剂杀螨效果较优,大大优于传统物理杀螨剂矿物油。同时,结果表明纳米颗粒粒径在185~200、330~380 nm区间的制剂造成柑橘全爪螨死亡的主要原因是通过气管堵塞的生物作用机制,而粒径在710~780 nm区间的纳米制剂主要是通过溶解虫体表面角质层造成柑橘全爪螨脱水死亡。[结论]由于羟丙基甲基纤维素对人体无毒无害,不会造成农残和对生态环境污染。若其代替有机合成农药应用于柑橘全爪螨虫害管理,拥有价格和环境保护的潜在优势,具有广阔的应用发展前景。[Aims]This study aims to investigate the impact of particle size of hydroxypropyl methylcellulose nano-formulations on the acaricidal efficacy against the citrus red spider mite.[Methods]Hydroxypropyl methylcellulose was utilized as the base material to prepare three distinct nanoparticle formulations with particle sizes of 185-200,330-380 and 710-780 nm,respectively,through high-speed mechanical shearing and ultrasonic dispersion techniques.The biological mechanisms underlying the mortality of the red spider mite were examined using laser confocal microscopy,metallurgical microscopy and electron scanning microscopy.[Results]All three nano-formulations demonstrated significant acaricidal activity against the citrus red spider mite.Notably,the formulation with a particle size of 330-380 nm exhibited superior acaricidal efficacy compared to the traditional physical acaricide,mineral oil.Additionally,the results indicated that the primary cause of mite mortality for the formulations with particle sizes of 185-200 and 330-380 nm was tracheal blockage,whereas the nano-formulation with a particle size of 710-780 nm primarily caused mite death through dehydration by dissolving the cuticle on the mite's surface.[Conclusions]Given that hydroxypropyl methylcellulose is non-toxic and poses no harm to human health,its use does not result in agricultural residues or environmental pollution.If utilized as an alternative to organic synthetic pesticides for red spider mite management,it will demonstrate potential advantages in terms of price and environmental protection,thereby presenting broad prospects for application and development.

关 键 词：羟丙基甲基纤维素 物理杀螨剂 纳米颗粒 柑橘全爪螨 生物作用机制 

分 类 号：TQ450.6[化学工程—农药化工]