4种柳属植物叶片差异表达基因分析  

Analysis on Differentially Expressed Genes in Leaves of Four Salix Species

作　　者：杜少波 姜迪 鄂崇毅 谢惠春 DU Shaobo;JIANG Di;E Chongyi;XIE Huichun(College of Geographical Sciences,Qinghai Normal University,Xining 810008,China;Qinghai south of Qilian Mountain Forest Ecosystem Observation and Research Station,Xining 810008,China;Academy of Life Science,Qinghai Normal University,Xining 810008,China)

机构地区：[1]青海师范大学地理科学学院,青海西宁810008 [2]青海祁连山南坡森林生态系统国家定位观测研究站,青海西宁810008 [3]青海师范大学生命科学学院,青海西宁810008

出　　处：《高原科学研究》2025年第1期30-42,共13页Plateau Science Research

基　　金：昆仑英才·高端创新创业人才计划特色人才项目“领军人才”(1003-005024011);青海省自然科学基金面上项目(2023-ZJ-908M)。

摘　　要：为探究腹毛柳(Salix delavayana)、坡柳(Salix myrtillacea)、康定柳(Salix paraplesia)和乌柳(Salix cheilophila)叶片的关键代谢途径和差异表达基因。该研究提取了以上4种柳属植物叶片总RNA后构建cD-NA文库,采用转录组测序技术进行测序,基于NR、GO和KEGG等数据库进行注释和功能富集分析,并探讨4种柳属植物间的差异表达基因和主要代谢途径。结果发现,在4种柳属植物种共检测到245994个Unigene,NR数据库注释结果表明,与小垫柳(Salix brachista)的同源性最高;GO和KEGG数据库注释结果发现,4种柳属植物主要参与细胞过程、代谢过程、细胞结构体、结合以及与信号转导功能和与代谢相关的功能;腹毛柳与坡柳、康定柳和乌柳的差异基因数量分别为34436条、49756条、87442条,坡柳与康定柳和乌柳的差异基因数量分别为51686条和89806条,康定柳和乌柳的差异基因数量为100423条;差异基因显著富集于植物病原体相互作用、植物昼夜节律、光合作用、蛋白水解、ABC转运蛋白和植物激素信号转导等二级代谢途径。研究结果可为进一步探究柳属植物的适应性机制提供理论参考。To investigate the Key metabolic pathways and differentially expressed genes in the leaves of Salix delavayana,S.myrtillacea,S.paraplesia,and S.cheilophila.This study extracted the total RNA from 4 species of Salix leaves and constructed a cDNA library using transcriptome sequencing technology for sequencing.The annotation and functional enrichment analysis were performed based on databases such as NR,GO,and KEGG,and explore the differentially expressed genes and main metabolic pathways among four species of Salix.A total of 245 994 unigenes were detected in four species of Salix.The annotation results of the NR database indicate that Salix brachista has the highest homology.The annotation results of GO and KEGG databases found that the four species of Salix mainly participate in cellular processes,metabolic processes,cell structures,binding,as well as functions related to signal transduction and metabolism.The number of differentially expressed genes between Salix delavayana and S.myrtillacea,and between Salix delavayana and S.paraplesia,and between Salixdelavayana and S.cheilophila is 34 436,49 756,and 87 442,respectively.The number of differentially expressed genes between Salix myrtillacea and S.paraplesia,and between Salix myrtillacea and S.cheilophila,is51 686 and 89 806,respectively.The number of differentially expressed genes between Salix paraplesia and S.cheilophila is 100 423.Differential genes are significantly enriched in plant pathogen interactions,plant circadian rhythms,photosynthesis,protein hydrolysis secondary metabolic pathways such as ABC transporter proteins and plant hormone signal transduction.The results of this study can provide theoretical reference for further exploring the adaptive mechanisms of Salix.

关 键 词：转录组测序技术 柳属植物 差异表达基因 代谢途径 

分 类 号：S792.99[农业科学—林木遗传育种]