蓝草类药材基原植物叶片表皮显微结构研究  

Study on leaf epidermal microstructure of medicinal blue herbs

作　　者：白云俊 赵玉洋 金艳[1] 付璐 袁媛[3] BAI Yunjun;ZHAO Yuyang;JIN Yan;FU Lu;YUAN Yuan(State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;China Institute for History of Chinese Medicine and Medical Literature,China Academy of Chinese Medical Sciences,Beijing 100700,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药资源中心,道地药材品质保障与资源持续利用全国重点实验室,北京100700 [2]中国中医科学院中国医史文献研究所,北京100700 [3]中国中医科学院医学实验中心,北京100700

出　　处：《药学实践与服务》2025年第4期174-179,共6页Journal of Pharmaceutical Practice and Service

基　　金：国家重点研发计划(2022YFC3500901);国家自然基金面上项目(82274053);中国中医科学院科技创新工程项目(CI2023E002,CI2021A04001)。

摘　　要：目的蓝草类药材复杂的基原演变历程可能导致混伪品的出现,影响临床用药的准确性。该文为叶片入药的蓝草类药材提供叶表皮显微结构鉴定依据。方法系统整理蓝草类药材所涉及的物种及其近缘种(4属10个物种),利用光学显微镜和扫描电子显微镜对其叶表皮显微结构进行观察分析,建立了基于显微结构的物种检索表。结果综合气孔器分布位置、副卫细胞类型、气孔指数、气孔密度、表皮细胞垂周壁形态以及表皮附属物等特征可准确区分大青属大青,蓼属蓼蓝,菘蓝属菘蓝、宽翅菘蓝、三肋菘蓝和小果菘蓝,马蓝属翅柄马蓝、曲枝假蓝、球花马蓝和板蓝等物种。结论叶表皮显微特征可准确区分蓝草类叶类药材的基原。Objective The complex evolutionary history of the origin of medicinal blue herbs might result in the presence of adulterants,affecting the accuracy and safety of clinical medication.To provide a reference basis for the identification of medicinal blue herbs with leaves as the medicinal part,based on the leaf epidermis microstructure.Methods The species belonging to medicinal blue herbs and their close relatives(10 species from 4 genera)were systematically investigated.The leaf epidermis microstructure of these species was observed and analyzed by optical microscopy and scanning electron microscopy.A species retrieval table was established based on the microstructure characteristics.Results By combining the distribution of stomata,types of subsidiary cells,stomatal index,stomatal density,characteristics of the periclinal walls of epidermal cells,and epidermal appendages,the species Clerodendrum cyrtophyllum,Polygonum tinctorium,Isatis indigotica,I.violascens,I.costata,I.minima,Strobilanthes wallichii,S.dalzielii,S.pentstemonoides,and S.cusia can be accurately distinguished.Conclusion Microscopic characteristics of leaf epidermis can provide reference data for accurately differentiating the botanical origins of medicinal blue herbs.

关 键 词：蓝草类药材 叶片 显微结构 物种鉴定 

分 类 号：R282.5[医药卫生—中药学]