表面增强拉曼光谱法快速测定尿液中曲马多的研究  

Rapid determination of tramadol in urine by surface-enhanced Raman spectroscopy

作　　者：姚小静 计佩影 陆峰 施国荣 傅翔 YAO Xiaojing;JI Peiying;LU Feng;SHI Guorong;FU Xiang(Shanghai Yangpu District Kongjiang Hospital,Shanghai 200082,China;Department of Pharmaceutical Analysis,School of Pharmacy,Naval Medical University,Shanghai 200433,China;Shanghai Yangpu District Shidong Hospital,Shanghai 200438,China)

机构地区：[1]上海市杨浦区控江医院,上海200082 [2]海军军医大学药学系药物分析教研室,上海200433 [3]上海市杨浦区市东医院,上海200438

出　　处：《药学实践与服务》2025年第4期185-189,共5页Journal of Pharmaceutical Practice and Service

基　　金：杨浦区科学技术委员会杨浦区卫生健康委员会科研课题(面上)(YPM202108);杨浦区科学技术委员会杨浦区卫生健康委员会科研课题(青年)(YPQ202114)。

摘　　要：目的应用液液萃取(LLE)-表面增强拉曼光谱(SERS),建立快速检测尿液中曲马多的方法。方法以氯仿∶异丙醇(9∶1)萃取剂从尿液中提取曲马多,采用增强拉曼光谱(波长为785 nm)检测尿液样品中的曲马多。结果曲马多的定量曲线Y=204.35 X-465.62,r=0.9952,线性范围为1~100μg/ml,该方法曲马多的检测限(S/N=3)为0.53μg/ml;与常规方法相比,SERS具有高灵敏度及合理的重现性。结论此方法简单、高效、经济,可以用于曲马多个性化用药的定性和定量分析。Objective To establish a method for rapid detection of tramadol in urine by liquid-liquid extraction(LLE)-surface-enhanced Raman spectroscopy(SERS).Methods Tramadol was extracted from urine with chloroform∶isopropyl alcohol(9∶1)extractant and detected in urine samples by enhanced Raman spectroscopy(wavelength 785 nm).Results The quantitative curve of tramadol was Y=204.35 X-465.62,r=0.9952,and the linear range was 1-100μg/ml.The detection limit of tramadol by this method(S/N=3)was 0.53μg/ml.The sensitivity of SERS was higher than that of conventional methods,and it had reasonable reproducibility.Conclusion This method is simple,efficient and economical,and can be used for qualitative and quantitative analysis of tramadol personalized medicine.

关 键 词：曲马多 尿液 表面增强拉曼光谱 潜在不适当用药 

分 类 号：R927.2[医药卫生—药学]