Realized heritability,cross-resistance patterns,and mechanisms of resistance to thiotraniliprole in Plutella xylostella(L.)  

小菜蛾对硫虫酰胺抗性的现实遗传力、交互抗性及其抗性机制

作　　者：YUAN Jing JIANG Yaqin CHANG Hui LEI Qiyang OUYANG Mingxun CHEN Yingchong CHEN Jing CHEN Jie 袁静;江雅琴;常慧;雷启阳;欧阳铭勋;陈滢冲;陈静;陈杰(浙江农林大学林业与生物技术学院,杭州311300;浙江长三角化学品安全评价有限公司,杭州311300;湖南环境生物职业技术学院,湖南衡阳421200)

机构地区：[1]College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China [2]Zhejiang Changsanjiao Chemical Safety Evaluation Co.,Ltd.,Hangzhou 311300,China [3]Hunan Polytechnic of Environment and Biology,Hengyang 421200,Hunan Province,China

出　　处：《农药学学报》2025年第2期311-321,共11页Chinese Journal of Pesticide Science

基　　金：Supported by the Zhejiang Provincial Public Welfare Technology Application Research Program(No:LGN21C140001).

摘　　要：Plutella xylostella,a major pest of cruciferous vegetables worldwide,has developed resistance to diamide insecticides.Thiotraniliprole,a novel synthetic diamide insecticide,exhibits excellent activity against P.xylostella.In the present study,we aimed to confirm the resistance risk,cross-resistance,and mechanisms of resistance to thiotraniliprole in P.xylostella.After 40 consecutive generations of thiotraniliprole selection,we obtained a thiotraniliprole-resistance P.xylostella strain with a 5141.58-fold resistance ratio(RR)to thiotraniliprole.The overall realized heritability(h^(2))value of resistance was estimated as 0.9 using threshold trait analysis,indicating that the risk of developing resistance to thiotraniliprole is high in P.xylostella.The thiotraniliprole-resistant(TR)strain showed noticeable cross-resistance to chlorantraniliprole(RR=44670.05),cyantraniliprole(RR=7038.58),and tetrachlorantraniliprole(RR=1506.01),but no cross-resistance to tolfenpyrad,indoxacarb,diafenthiuron,or abamectin compared with the susceptible(S)strain.The enzyme assay data showed that the activities of glutathione-S transferase(GST),carboxylesterase(CarE),and the content of cytochrome P450 monooxygenase(P450s)were significantly higher in the TR strain than in the S strain.Sequencing of the full-length PxRyR cDNA revealed the gene site I4790K in the TR strain with a 100%frequency.This mutation in PxRyR likely underlies the high-level cross-resistance between thiotraniliprole and three other diamide insecticides.These findings provide valuable information for optimizing resistance management strategies to delay thiotraniliprole resistance development and ensure sustainable control of P.xylostella.小菜蛾是世界范围内重要的十字花科蔬菜害虫,硫虫酰胺是新型双酰胺类杀虫剂,对小菜蛾具有优异活性,本研究旨在研究小菜蛾对硫虫酰胺的抗性风险、交互抗性及其抗性机制。以硫虫酰胺对小菜蛾连续汰选40代,获得抗硫虫酰胺品系,其抗性倍数(RR)为5141.58倍,现实遗传力(h^(2))为0.9,表明小菜蛾对硫虫酰胺为高抗性风险。硫虫酰胺与氯虫苯甲酰胺(RR=44670.05)、溴氰虫酰胺(RR=7038.05)、四氯虫酰胺(RR=1506.01)存在显著交互抗性;与唑虫酰胺、茚虫威、丁醚脲和阿维菌素均无交互抗性。抗硫虫酰胺小菜蛾品系解毒酶GST、P450s和CarE的活性均显著高于敏感品系的。对抗硫虫酰胺小菜蛾PxRyR cDNA全长进行测序并比对分析发现,I4790K氨基酸位点突变频率为100%,这可能是与其他3种双酰胺类杀虫剂存在交互抗性的主要原因。该研究对延缓小菜蛾对硫虫酰胺的抗性,指导其抗性治理具有重要意义。

关 键 词：Plutella xylostella thiotraniliprole realized heritability CROSS-RESISTANCE detoxifying enzymes ryanodine receptor resistance mechanisms 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] S482.3[农业科学—植物保护]