CNTs/CNFs/GCE电化学传感器的制备及其对木犀草素的检测  

Preparation of CNTs/CNFs/GCE electrochemical sensor for the detection of luteolin

作　　者：朱旭文 郭双 李春婷 张丽文 程昊[1,2,3] 唐婷范 ZHU Xuwen;GUO Shuang;LI Chunting;ZHANG Liwen;CHENG Hao;TANG Tingfan(Guangxi Key Laboratory of Green Processing of Sugar Resources(Guangxi University of Science and Technology),Liuzhou 545006,China;Guangxi Liuzhou Luosifen Research Center of Engineering Technology(Guangxi University of Science and Technology),Liuzhou 545006,China;School of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,China;Hangzhou Weinian Brand Management Co.,Ltd.,Hangzhou 310000,China)

机构地区：[1]广西糖资源绿色加工重点实验室(广西科技大学),广西柳州545006 [2]广西柳州螺蛳粉工程技术研究中心(广西科技大学),广西柳州545006 [3]广西科技大学生物与化学工程学院,广西柳州545006 [4]杭州微念品牌管理有限公司,浙江杭州310000

出　　处：《广西科技大学学报》2025年第2期118-127,共10页Journal of Guangxi University of Science and Technology

基　　金：广西自然科学基金面上项目(2022GXNSFAA035490);广西自然科学基金项目(2025GXNSFHA069196);广西高校桂菜工业化加工与安全重点实验室开放基金项目(2024KLGC0204)资助。

摘　　要：以碳纳米管(carbon nanotubes,CNTs)和聚丙烯腈(polyacrylonitrile,PAN)为前驱体,通过静电纺丝得到纳米纤维,后经碳化得到CNTs/CNFs复合材料。使用扫描电子显微镜(scanning electron microscope,SEM)、X射线衍射(X-ray diffraction,XRD)、能量色谱仪(energy dispersive spectroscopy,EDS)和电化学阻抗谱(electrochemical impedance spectroscopy,EIS)对其形貌和组成进行了表征;以滴涂法将此材料修饰到玻碳电极(glassy carbon electrode,GCE)表面,考察木犀草素在该复合电极上的电化学行为;最后将此修饰电极应用到金银花露中木犀草素的测定。结果表明:此修饰电极在检测木犀草素的试验中展现出良好的重复性、重现性和抗干扰能力;当木犀草素浓度在3~10μmol/L时,CNTs/CNFs/GCE修饰电极响应电流值与木犀草素浓度呈良好的线性关系,检测限为0.18μmol/L;用此复合电极检测金银花露中的木犀草素,加标回收率在96.069%~97.623%之间,相对标准偏差小于5.0%。To prepare an electrochemical sensor for the detection of luteolin,carbon nanotubes(CNTs)and polyacrylonitrile(PAN)were used as precursors to yield nanofibers by electrostatic spinning,followed by carbonization to obtain CNTs/CNFs composites.The morphology and composition were characterized using scanning electron microscopy(SEM),X-ray diffraction(XRD),energy dispersive spectroscopy(EDS)and electrochemical impedance spectroscopy(EIS).This material was modified onto the surface of a glassy carbon electrode(GCE)by drop-coating method.The electrochemical behavior of luteolin on this composite electrode was investigated,and finally the sub-modified electrode was applied to the determination of luteolin in Aqua lonicerae foliae.This modified electrode demonstrated good repeatability,reproducibility and immunity to interference in the assay for the detection of lignocaine.The CNTs/CNFs/GCE-modified electrode showed a good linear relationship between the response current value and luteolin concentration with a detection limitation of 0.18μmol/L when the luteolin concentration was in the range of 3 to 10μmol/L.The luteolin in Aqua lonicerae foliae was detected by this composite electrode and the spiked recoveries ranged from 96.069%to 97.623%with RSD<5.0%.

关 键 词：木犀草素 碳纳米管 静电纺丝法 电化学 加标回收率 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]