福建柏不定芽生根诱导及其内源激素研究  

Induction of Adventitious Bud Rooting and Its Endogenous Hormones in Fokienia hodginsii

作　　者：杨丹丹[1] 王小妹 卢冰冰 王雨溪 荣俊冬[2] 何天友[1] 陈礼光[2] 郑郁善[1] YANG Dandan;WANG Xiaomei;LU Bingbing;WANG Yuxi;RONG Jundong;HE Tianyou;CHEN Liguang;ZHENG Yushan(College of Landscape Architecture and Art,Fujian Agriculture and Forestry University,Fuzhou Fujian 350000;College of Forestry,Fujian Agriculture and Forestry University,Fuzhou Fujian 350000)

机构地区：[1]福建农林大学风景园林与艺术学院,福建福州350000 [2]福建农林大学林学院,福建福州350000

出　　处：《山东林业科技》2025年第1期1-8,14,共9页Journal of Shandong Forestry Science and Technology

基　　金：福建省林业种苗科技攻关七期项目(LZKG-202207);福建农林大学科技创新专项基金项目(KFB23057)。

摘　　要：为建立福建柏不定芽诱导生根过程中最适激素配比及探究诱导生根过程中外源激素对内源激素的影响,取福建柏上当年生幼嫩无害的带芽茎段进行组织培养,以所获得的不定芽作为研究对象,测定不同配方下不定芽的生根率、污染率及内源激素的变化情况。结果表明:单独添加1.0 mg·L^(-1)IBA、添加0.5mg·L^(-1)IBA+0.1 mg·L^(-1)NAA或者0.5 mg·L^(-1)IBA+0.1 mg·L^(-1)IAA能诱导不定芽生根;最佳生长素组合为1.0 mg·L^(-1)IBA+0.1 mg·L^(-1)NAA;最佳生长调节剂组合为0.1 mg·L^(-1)6-BA+0.5 mg·L^(-1)IBA;生根粉对不定芽诱导生根无明显作用;最佳诱导生根活性炭浓度为0.2 g/L;最佳诱导生根蔗糖浓度为30 g/L;诱导生根过程中,GA3、IAA、ABA激素含量有明显变化,CTK含量变化较小。研究初步建立了福建柏组培苗最佳生根激素配比,探究了内源激素变化,可为工厂化育苗提供技术支持。In order to determine the optimal hormone ratios and the effects of exogenous and endogenous hormones during the induced rooting of adventitious shoots of Fokienia hodginsii.The adventitious shoots were obtained from young and healthy stem segments of the current year sourced from Fujian cypress plants and cultured in tissue culture.The rooting rate of adventitious shoots,the contamination rate,and the changes of endogenous hormones in the plants were observed and measured under different conditioning formulas.The results showed that supplementation of 1.0 mg·L^(-1)IBA alone,0.5 mg·L^(-1)IBA+0.1 mg·L^(-1)NAA,or 0.5 mg·L^(-1)IBA+0.1 mg·L^(-1)IAA in combination could induce rooting of adventitic buds.The optimal auxin combination was 1.0 mg·L^(-1)IBA+0.1 mg·L^(-1)NAA,while the optimal plant growth regulator combination was 0.1 mg·L^(-1)6-BA+0.5 mg·L^(-1)IBA.The root powder had no obvious effect on the induced rooting of adventitious buds,and direct dipping in root powder increased the contamination rate.The optimal concentration of activated carbon was 0.2g,and the optimal rooting sucrose concentration was 30g/L.During the induction of rooting,the contents of GA3,IAA and ABA were significantly altered,while the contents of cytokinins(CTK)changed little.This study has preliminarily determined the optimal hormone ratios for the rooting of F.hodginsii tissue-culture seedlings,investigated the changes in endogenous hormones,and can thus offer technical support for industrialized nursery cultivation.

关 键 词：福建柏 不定芽 生根 内源激素 

分 类 号：S791.43[农业科学—林木遗传育种]