Ultra-sensitive magnetic nanomechanical array sensor based on graphene oxide for single bacterial cell detection  

作　　者：Zihan Qiao Kainan Mei Yu Wang Tianhao Yan Shubo Zhang Qiubo Chen Ye Chen Chen Wang Tianxiang Ren Shangquan Wu Qingchuan Zhang 

机构地区：[1]CAS Key Laboratory of Mechanical Behavior and Design of Material,Department of Modern Mechanics,University of Science and Technology of China,Hefei 230027,China [2]Department of Cell Biology and Genetics,College of Basic Medical Sciences,Jilin University,Changchun 130021,China [3]School of Mechanical and Aerospace Engineering,College of Engineering,Nanyang Technological University,Singapore 639798,Singapore [4]Institute of Flexible Electronics Technology of THU,Jiaxing 314000,China [5]State Key Laboratory of Nonlinear Mechanics,Institute of Mechanics,Chinese Academy of Science,Beijing 100190,China [6]Hefei National Research Center for Physical Sciences at the Microscale,Department of Chemical Physics,University of Science and Technology of China,Hefei 230026,China

出　　处：《Nano Research》2025年第4期411-424,共14页纳米研究(英文版)

基　　金：the National Natural Science Foundation of China(Nos.12232017,12222212,and 12072339)is gratefully acknowledged.

摘　　要：Pathogenic bacterial infections pose major health threats and economic burdens.Rapid and highly sensitive biochemical sensors are essential for bacterial detection in food safety and clinical applications.Here,we introduce a graphene oxide(GO)-based magnetic nanomechanical array sensor that utilizes the large surface area of GO to bind more magnetic nanoparticles(MNPs)and aptamers.Rapid and ultra-sensitive detection can be achieved even at extremely low target concentrations.This approach can directly detect a single Escherichia coli cell without time-consuming bacterial culture,and the linear detection range is 1-100 CFU·mL-1.Meanwhile,the sensor showed good specificity,reproducibility,stability,and stance to interference,and could detect 1 CFU·mL^(-1) Escherichia coli in milk.Moreover,we realized the simultaneous detection of two bacteria at extremely low concentrations,which proved that the sensor had the potential for high-throughput detection.In addition,for extremely low-concentration samples(<100 CFU·mL^(-1)),we controlled the magnetic force at the tip of the microcantilever,greatly enhancing its deflection and sensitivity.This method provides a novel and ultrasensitive method for the timely detection of pathogenic bacteria,and can also be applied to the highly sensitive detection of other targets such as DNA,small molecules,proteins,and viruses by using different probes.Our research provides a promising tool for effective,rapid and highly sensitive detection in the field of public health and food safety.

关 键 词：bacterial detection microcantilever array graphene oxide magnetic force nanomechanical sensor 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]