山新杨PdaYABBY1基因克隆及表达分析  

Cloning and expression analysis of PdaYABBY1 gene in Populus davidiana×P.alba var.pyramidalis

作　　者：陈彤 董心仪 高向博 朱祯煜 王超[1] CHEN Tong;DONG Xinyi;GAO Xiangbo;ZHU Zhenyu;WANG Chao(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China)

机构地区：[1]东北林业大学林木遗传育种全国重点实验室,哈尔滨150040

出　　处：《植物生理学报》2025年第3期337-347,共11页Plant Physiology Journal

基　　金：科技创新2030-重大项目(2023ZD0405603);黑龙江省大学生创新创业训练项目(S202410225036)。

摘　　要：植物特异性转录因子家族YABBY具有典型的N端C2C2型锌指结构域和C端YABBY保守结构域,在植物生长发育和形态发生中起着重要的作用。尽管YABBY的功能已在多种物种中已有广泛研究,但其在杨树中的研究仍然相对缺乏。本研究克隆得到山新杨的YABBY基因PdaYABBY1,其ORF全长633 bp,编码210个氨基酸。PdaYABBY1具有典型的YABBY蛋白结构特征,含有2个保守基序,由α螺旋和β折叠形成蛋白三级结构,定位于细胞核中。系统进化树分析表明,PdaYABBY1与毛果杨(Populus trichocarpa)和葡萄(Vitis vinifera)的YABBY蛋白亲缘关系较近。实时荧光定量PCR结果表明,PdaYABBY1基因在叶片中表达量较高,而在根部表达量较低。此外,PdaYABBY1响应盐胁迫与干旱胁迫,干旱胁迫下,其在根部的表达量显著增加,而在盐胁迫下,其在叶片中的表达量显著增加。这些结果表明PdaYABBY1可能在山新杨的抗旱和耐盐过程中起着重要作用。The plant-specific YABBY transcription factor family,with a typical N-terminal C2C2-type zinc finger structural domain and a C-terminal YABBY conserved structural domain,plays an important role in plant growth,development,and morphogenesis.Despite extensive research on the function of YABBY in a variety of species,there is still a paucity of studies on this topic in poplar.In this study,we cloned PdaYABBY1,a YABBY gene from Populus davidiana×P.alba var.pyramidalis with a full ORF length of 633 bp,encoding 210 amino acids.PdaYABBY1 exhibits the typical structural features of YABBY proteins,containing two conserved motifs,forming a protein tertiary structure byα-helix andβ-folding,and localizing in the nucleus.Phylogenetic tree analysis demonstrated that PdaYABBY1 was closely related to the YABBY proteins of P.trichocarpa and Vitis vinifera.RT-qPCR results showed that the expression of the PdaYABBY1 gene was higher in leaves and lower in roots.Furthermore,PdaYABBY1 demonstrated responsiveness to salt and drought stress.The expression of PdaYABBY1 in roots exhibited a notable increase under drought stress,whereas its expression in leaves demonstrated a significant elevation under salt stress.These findings suggest that PdaYABBY1 may play a pivotal role in drought and salt tolerance in P.davidiana×P.alba var.pyramidalis.

关 键 词：YABBY 山新杨 基因表达 非生物胁迫 

分 类 号：S792.11[农业科学—林木遗传育种]