丹酚酸B基于JAK2/STAT3通路对大肠埃希菌感染肾盂肾炎大鼠的干预效果  

Intervention effect of salvianolic acid B based on JAK2/STAT3 pathways on rats with Escherichia coli infection-induced pyelonephritis

作　　者：张玲[1] 王志芳[1] 姜金孝 漆晨 黄古飞 ZHANG Ling;WANG Zhifang;JIANG Jinxiao;QI Chen;HUANG Gufei(Huanggang Central Hospital,Huanggang,Hubei 438000,China)

机构地区：[1]黄冈市中心医院肾病学科,湖北黄冈438000

出　　处：《中华医院感染学杂志》2025年第6期807-811,共5页Chinese Journal of Nosocomiology

基　　金：湖北省自然科学基金青年基金资助项目(2023AFB442);湖北科技学院医学部教学改革研究基金资助项目(2023YJDJY12)。

摘　　要：目的分析丹酚酸B基于JAK2/STAT3通路对大肠埃希菌感染肾盂肾炎大鼠的干预效果。方法选取60只SPF级SD雄性大鼠,按照完全随机分组分为对照组21只、模型组(大肠埃希菌感染肾盂肾炎)18只和丹酚酸B组(大肠埃希菌感染肾盂肾炎+丹酚酸B)18只,对照组及模型组取同等体积生理盐水灌胃,丹酚酸B组给予12.5 mg/kg丹酚酸B水溶液灌胃,检测各组大鼠相关生化指标[分泌型免疫球蛋白A(SlgA)、尿肌酐和血清肌酐等]水平,比较各组脏器指数及JAK2/STAT3信号通路相对蛋白表达情况。结果各组大鼠血液指标水平比较,差异均有统计学意义(P<0.05),与对照组SlgA水平[(25.64±4.51)μg/ml]相比,模型组[(13.51±1.18)μg/ml]降低(P<0.05),与模型组相比,丹酚酸B组SlgA水平[(25.34±2.95)μg/ml]上升(P<0.05)。三组膀胱指数比较,无统计学差异;模型组左右肾比值、肾脏指数高于对照组(P<0.05);丹酚酸B组左右肾比值、肾脏指数均低于模型组(P<0.05)。各组大鼠JAK2/STAT3信号通路相对蛋白表达比较,差异有统计学意义(P<0.05),与对照组相比,模型组JAK2、STAT3水平较高(P<0.05);与模型组相比,丹酚酸B组JAK2、STAT3水平均呈现较低表达(P<0.05)。结论丹酚酸B经JAK2/STAT3通路对大肠埃希菌感染肾盂肾炎大鼠各项指标水平进行改善,有效改善大鼠整体症状。OBJECTIVE To observe intervention effect of salvianolic acid B based on JAK2/STAT3 pathways on the rats with Escherichia coli infection-induced pyelonephritis.METHODS Totally 60 SPF grade male SD rats were included in the study and were randomly divided into the control group with 21 rats,the model group(Escherichia coli infection-induced pyelonephritis)with 18 rats and the salvianolic acid B group(Escherichia coli infection-induced pyelonephritis+salvianolic acid B)with 18 rats.The control group and the model group were treated with same volume of normal saline for gavage,the salvianolic acid B group was given 12.5 mg/kg salvianolic acid B water solution for gavage.The levels of related biochemical indexes[secretory immunoglobulin A(SlgA),urine creatinine and serum creatinine]were detected for the groups.The organ indexes and relative expression levels of JAK2/STAT3 signaling pathways were observed and compared among the groups.RESULTS There were significant differences in the levels of blood indexes among the three groups of rats(P<0.05).The SlgA level of the model group was(13.51±1.18)μg/ml,lower than(25.64±4.51)μg/ml of the control group(P<0.05);the SlgA level of the salvianolic acid B group was(25.34±2.95)μg/ml,higher than that of the model group(P<0.05).There were no significant differences in the bladder indexes among the three groups;the ratio of left kidney to right kidney and the renal indexes were higher in the model group than in the control group(P<0.05);the ratio of left kidney to right kidney and the renal indexes were lower in the salvianolic acid B group than in the model group(P<0.05).There were significant differences in the relative expression levels of JAK2/STAT3 signaling pathways among the three groups(P<0.05);the levels of JAK2 and STAT3 of the model group were higher than those of the control group(P<0.05);the levels of JAK2 and STAT3 of the salvianolic acid B group were lower than those of the model group(P<0.05).CONCLUSION The salvianolic acid B based on JAK2/STAT3 pathways may im

关 键 词：丹酚酸B 蛋白酪氨酸激酶-2/信号转导和转录激活因子-3 大肠埃希菌 肾盂肾炎 感染 

分 类 号：R256.5[医药卫生—中医内科学]