耐碳青霉烯高毒力肺炎克雷伯菌血流感染小鼠血清蛋白组学分析  

Proteomic analysis of serum proteins in mice with carbapenem-resistant hypervirulent Klebsiella pneumoniae bloodstream infection

作　　者：鲍金凤 麻雅婷 徐茵[1] 何燕娟[1] 王玉月[1] 时洛洛 王成彬[2] BAO Jinfeng;MA Yating;XU Yin;HE Yanjuan;WANG Yuyue;SHI Luoluo;WANG Chengbin(The First People's Hospital of Changzhou,Changzhou,Jiangsu 213003,China;不详)

机构地区：[1]常州市第一人民医院检验科,江苏常州213003 [2]解放军总医院第一医学中心检验科,北京100853 [3]北京医院国家老年中心,国家卫生健康委临床检验中心,中国医学科学院老年医学研究院,北京100730

出　　处：《中华医院感染学杂志》2025年第6期823-828,共6页Chinese Journal of Nosocomiology

基　　金：中央高水平医院临床科研业务费基金资助项目(BJ-2021-201);常州市卫健委青年人才科技项目基金资助项目(QN202214,QN202302)。

摘　　要：目的应用液相色谱-质谱仪(LC-MS)分析耐碳青霉烯高毒力肺炎克雷伯菌(CR-hvKP)血流感染小鼠的血清蛋白变化,寻找差异表达蛋白,并进行相应的生物学功能分析。方法建立ICR小鼠CR-hvKP及经典肺炎克雷伯菌(cKP)血流感染模型,收集感染12 h小鼠血清,进行LC-MS检测。采用Maxquant软件对LC-MS结果进行鉴定,并对差异蛋白进行相应的生物信息学分析。结果与正常对照组相比,CR-hvKP组分别有24个蛋白上调和20个蛋白下调。与cKP组相比,CR-hvKP组有107个蛋白上调。134个差异表达蛋白于Uniprot数据库逐一进行检索,发现这些蛋白参与生物调控、免疫过程、生物间相互作用、运动、代谢过程、对刺激的反应、信号转导、炎症反应、氧化应激、血管生成等过程。信号通路分析涉及补体和凝血级联、胆固醇代谢、细菌感染、血红素清除、血浆脂蛋白组装、重塑和清除等多条代谢相关通路。重点关注蛋白,其中激肽释放酶B1(KLKB1)及丝氨酸蛋白酶抑制剂家族A成员(Serpina)1b与正常组相比下调但与cKP组相比上调,淀粉样蛋白A(SAA)1、SAA2、泛酰巯乙胺酶3(VNN3)、血红蛋白β多肽链b2(HBB-b2)蛋白与cKP组及正常组相比均上调。结论CR-hvKP血流感染涉及多个蛋白的激活及作用,SAA1、SAA2、VNN3、HBB-b2升高或KLKB1、Serpina1b下降时或提示CR-hvKP血流感染。OBJECTIVE To observe the changes of serum proteins in the mice with carbapenem-resistant hypervirulent Klebsiella pneumoniae(CR-hvKP)bloodstream infection by using liquid chromatogram-mass spectrometer(LC-MS),find out the differentially expressed proteins and carry out corresponding biological function analysis.METHODS The models of ICR mice with CR-hvKP and classical Klebsiella pneumoniae(cKP)bloodstream infections were established,the serum specimens were collected from the mice at 12 hours of establishment of the infection models and were detected by using LC-MS.The result of LC-MS was identified by using Maxquant software.The corresponding bioinformatics analysis was performed for the differentially expressed proteins.RESULTS As compared with the normal control group,there were 24 upregulated proteins and 20 downregulated proteins in CR-hvKP group.As compared with cKP group,there were 107 upregulated proteins in the CR-hvKP group.The 134 differentially expressed proteins were retrieved one by one from Uniprot database,it was found that the proteins were involved in biological regulation,immune process,biological interaction,movement,metabolic process,response to stimulation,signal transduction,inflammatory response,oxidative stress and angiogenesis.The signal pathway analysis involved multiple metabolism-related pathways,including complement and coagulation cascades,cholesterol metabolism,bacterial infection,heme clearance,and assembly,remodeling and clearance of plasma lipoprotein.In terms of the proteins being attached great importance,the expression levels of kallikrein B1(KLKB1)and serpin family A(Serpina)1b were downregulated as compared with the normal control group and were upregulated as compared with the cKP group;the expression levels of serum amyloid A protein(SAA)1,SAA2,Vanin-3(VNN3)and hemoglobinβpolypeptide chain b2(HBB-b2)were upregulated as compared with both the cKP group and the normal control group.CONCLUSIONS The CR-hvKP bloodstream infection involves the activation and action of multiple

关 键 词：耐碳青霉烯 高毒力肺炎克雷伯菌 液相色谱-质谱 血流感染 血清淀粉样蛋白A 激肽释放酶B1 

分 类 号：R378.996[医药卫生—病原生物学]