儿童哮喘合并呼吸道病毒感染外周血miR-21、Th1/Th2细胞因子水平及其意义  

Expression levels of miR-21 and Th1/Th2 cytokines in peripheral blood of asthma children complicated with respiratory tract infections of viruses and their significance

作　　者：陈小桥[1] 任美玲 孙志伟[1] 薛新 施科 CHEN Xiaoqiao;REN Meiling;SUN Zhiwei;XUE Xin;SHI Ke(The 904th Hospital of The Joint Logistics Support Force of The People's Liberation Army of China,Wuri,Jiangsu 21410l,China)

机构地区：[1]中国人民解放军联勤保障部队第904医院儿科,江苏无锡214101

出　　处：《中华医院感染学杂志》2025年第6期904-908,共5页Chinese Journal of Nosocomiology

基　　金：江苏省自然科学基金资助项目(BK20221015)。

摘　　要：目的探讨儿童哮喘合并呼吸道病毒感染外周血微小RNA(miR-21)、辅助性T淋巴细胞(Th)1/Th2细胞因子水平的表达及意义。方法收集2021年1月-2023年12月联勤保障部队第九〇四医院收治的90例呼吸道病毒感染的哮喘患儿(感染组)及43例无呼吸道病毒感染的哮喘患儿(无感染组)的临床资料,分析感染组感染病毒分布情况,并对两组患儿外周血miR-21、Th1细胞因子[γ干扰素(IFN-γ)、白细胞介素(IL)-2]、Th2细胞因子[IL-4、IL-5]进行比较;感染组患儿根据病情严重程度分为轻、中及重度组,比较不同病情严重程度患儿上述指标水平,分析患儿病情严重程度与miR-21、IFN-γ、IL-2、IL-4和IL-5的相关性。结果感染组呼吸道病毒检出以呼吸道合胞病毒为主,占32.22%。与无感染组相比,感染组IFN-γ及IL-2降低(P<0.05),miR-21、IL-4及IL-5升高(P<0.05);与轻及中度组相比,重度组IFN-γ及IL-2降低(P<0.05),而miR-21、IL-4及IL-5升高(P<0.05),且中度组IFN-γ及IL-2水平低于轻度组(P<0.05),miR-21、IL-4及IL-5水平高于轻度组(P<0.05);Spearman秩相关性分析显示,患儿病情严重程度与miR-21、IL-4及IL-5呈正相关(P<0.05),与IFN-γ及IL-2呈负相关(P<0.05)。结论呼吸道病毒感染会加剧哮喘患儿外周血miR-21水平异常上升及Th1/Th2失衡,且上述指标与患儿病情严重程度密切相关。OBJECTIVE To explore the expression levels of microribonucleic acid-21(miR-21)and helper T cell(Th)1/Th2 cytokines in peripheral blood of the asthma children complicated with respiratory tract infections of viruses and analyze the significance.METHODS A total of 90 asthma children with respiratory tract infections of viruses(the infection group)and 43 asthma children without respiratory tract infections of viruses(the no infection group)who were treated in the 904th Hospital of the Joint Logistics Support Force of The People′s Liberation Army of China were enrolled in the study,and the clinical data were collected from the enrolled patients.The distribution of viruses from the children of the infection group was analyzed.The levels of peripheral blood miR-21,Th1 cytokines[interferon-γ(IFN-γ),interleukin(IL)-2]and Th2 cytokines[IL-4,IL-5]were compared between the two groups.The children of the infection group were divided into the mild group,the moderate group and the severe group according to the severity of disease.The levels of the above indexes were compared among the children with the various degree of illness condition.The association of the illness condition with miR-21,IFN-γ,IL-2,IL-4 and IL-5 was analyzed.RESULTS Respiratory syncytial virus was dominant among the respiratory tract viruses in the infection group,accounting for 32.22%.The levels of IFN-γand IL-2 of the infection group were lower than those of the no infection group(P<0.05),the levels of miR-21,IL-4 and IL-5 of the infection group were higher than those of the no infection group(P<0.05).The levels of IFN-γand IL-2 of the severe group were lower than those of the mild group and the moderate group(P<0.05),while the levels of miR-21,IL-4 and IL-5 of the severe group were higher than those of the mild group and the moderate group(P<0.05);the levels of IFN-γand IL-2 of the moderate group were lower than those of the mild group(P<0.05),and the levels of miR-21,IL-4 and IL-5 of the moderate group were higher than those of the mild group(P<0

关 键 词：哮喘 呼吸道病毒感染 微小RNA-21 辅助性T细胞1 辅助性T细胞2 

分 类 号：R725.6[医药卫生—儿科]