细胞基质四价流感病毒亚单位疫苗配伍纳米乳佐剂在不同鼠龄段BALB/c小鼠体内的免疫原性  

Immunogenicity of cell-based quadrivalent influenza subunit vaccine with nano-emulsion adjuvant in different age groups of BALB/c mice

作　　者：家峥 王红红 刘莹 闫伊萌 李雪娇 郭丹丹 赵帅 吴业红 张雪梅 JIA Zheng;WANG Honghong;LIU Ying;YAN Yimeng;LI Xuejiao;GUO Dandan;ZHAO Shuai;WU Yehong;ZHANG Xuemei(Changchun Institute of Biological Products,Co.,Ltd.,Changchun 130012,Jilin Province,China;不详)

机构地区：[1]长春生物制品研究所有限责任公司,吉林长春130012 [2]新突发传染病新型疫苗研发全国重点实验室,北京100024 [3]武威市药品检验检测中心,甘肃武威733000 [4]北京生物制品研究所有限责任公司,北京100000

出　　处：《中国生物制品学杂志》2025年第3期264-271,278,共9页Chinese Journal of Biologicals

基　　金：吉林省科技发展计划项目(20210204082YY).

摘　　要：目的评价纳米乳佐剂(nano-emulsion adjuvant,NE)配伍细胞基质四价流感病毒亚单位疫苗(cell-based quadri-valent influenza subunit vaccine,QIVc)对小鼠体液免疫的增强作用。方法以NE(50μL)配伍QIVc,肌内注射免疫3周龄、6周龄、18月龄BALB/c雌性小鼠,免疫剂量为血凝素(hemagglutinin,HA)1.5μg/(株·只),2针免疫间隔28 d。初次免疫后28 d及末次免疫后14 d,检测小鼠血清中血凝抑制(hemagglutination inhibition,HI)效价和IgG抗体水平,分析3种鼠龄段小鼠的佐剂增强作用,同时设无佐剂及PBS对照组。末次免疫后14 d,以10倍病毒半数致死量(median lethal dose,LD_(50))B/Maryland/15/2016(B-Victoria)流感毒株进行攻毒试验,连续14 d观察小鼠体质量和生存率变化。结果NE联合HA肌内注射免疫可诱导比单独HA肌内注射免疫更高水平的血清HI、IgG抗体,在18月龄小鼠中的免疫增强作用更明显;NE能够增强HA的免疫原性以及小鼠对B/Maryland/15/2016(B-Victoria)流感毒株攻击的抵抗力。结论NE可增强流感抗原的免疫原性,且对老龄小鼠的增强作用更明显。Objective To evaluate the effect of cell-based quadrivalent influenza subunit vaccine(QIVc)with nano-emulsion adjuvant(NE)on the enhancement of humoral immunity in mice.Methods Three-week-old,six-week-old and 18-month-old BALB/c female mice were immunized with QIVc combined with NE(50μL)by intramuscular injection at the hemagglutinin(HA)dose of 1.5μg(plant·unit),for two injections with the interval of 28 d.The hemagglutination inhibition(HI)titers and IgG antibody levels in serum of mice were measured 28 d after the initial immunization and 14 d after the last immunization,and the adjuvant enhancement effects of three age groups of mice were analyzed.The adjuvant-free and PBS control groups were set up.Fourteen days after the last immunization,the mice were challenged with 10 folds of median lethal dose(LD_(50))B/Maryland/15/2016(B-Victoria)influenza strain,and the changes of body mass and survival rate were observed for 14 consecutive days.Results Intramuscular injection of HA combined with NE induced higher levels of serum HI and IgG antibodies than intramuscular injection of HA alone,and the immune-enhancing effect was more obvious in 18-month-old mice.NE enhanced the immunogenicity of HA and the resistance of mice to the attack of B/Maryland/15/2016(B-Victoria)influenza strain.Conclusion NE can enhance the immunogenicity of influenza antigen,and the enhancement effect on elderly mice is more obvious.

关 键 词：四价流感病毒亚单位疫苗 纳米乳佐剂 鼠 鼠龄 免疫原性 

分 类 号：R373.31[医药卫生—病原生物学]