响应面法优化老鹳草水提工艺及其抗炎作用研究  

Optimization of the aqueous extraction of Geranium wilfordii Maxim.and its anti-inflammation properties

作　　者：王美玲 兰卫[1] WANG Mei-ling;LAN Wei(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830017)

机构地区：[1]新疆医科大学中医学院,乌鲁木齐830017

出　　处：《中南药学》2025年第4期929-934,共6页Central South Pharmacy

基　　金：新疆乌鲁木齐自治区重大专项子课题(No.2022A03017-5-3)。

摘　　要：目的采用响应面法优化老鹳草水提工艺,探讨其对脂多糖(LPS)诱导的RAW264.7细胞炎症的抑制作用。方法以没食子酸、槲皮素含量为评价指标,采用AHP-熵权法计算权重系数,综合评价分析并结合Box-Behnken响应面考察提取温度、料液比、提取时间对老鹳草水提工艺的影响;CCK-8比色法检测老鹳草水提物对RAW264.7细胞增殖的抑制作用;不同浓度的水提物(250、500、1000μg·mL^(-1))干预体外培养LPS诱导的RAW264.7细胞24、48 h后,Griess法检测一氧化氮(NO)变化。结果最佳提取工艺条件:提取时间55 min、料液-1比1∶21(g/mL)、提取温度100℃。与模型组比较,250、500、1000μg·mL^(-1)的老鹳草水提物作用RAW264.7细胞24和48 h后,NO生成量均显著降低(P<0.0001)。结论通过响应面法优化得出的老鹳草水提工艺,经验证稳定合理、操作可控、重现性好,并发现老鹳草水提物能够显著抑制LPS诱导的RAW264.7炎症细胞的NO生成量。Objective To optimize the aqueous extraction of Geranium wilfordii Maxim.and determine its inhibition effect on lipopolysaccharide-induced inflammation in RAW264.7 cells with response surface methodology.Methods The content of gallic acid and quercetin was used as the evaluation indexes.The weight coefficient was calculated by AHP-entropy weight method,and the effects of extraction temperature,solid-liquid ratio and extraction time on the water extraction of Geranium wilfordii Maxim.were determined by comprehensive evaluation and analysis combined with Box-Behnken response surface method.The inhibition of aqueous extract of Geranium wilfordii Maxim.on the proliferation of RAW264.7 cells was detected by CCK-8 colorimetric method,and the nitric oxide change was detected by Griess method after different concentrations of aqueous extracts(250,500 and 1000μg·mL^(-1))were intervened in vitro to culture lipopolysaccharide-induced RAW264.7 cells for 24 and 48 h.Results The optimal extraction conditions were as follows:extraction for 55 min,solid-liquid ratio at 1∶21(g/mL),and extraction temperature 100℃.Compared with the model group,the amount of nitric oxide was significantly reduced after 24 and 48 h treatment of aqueous extracts at 250,500 and-11000μg·mL^(-1) in RAW264.7 cells(P<0.0001).Conclusion The aqueous extraction of Geranium wilfordii Maxim.by response surface optimization is stable,reasonable,controllable,and reproducible,and can inhibit the nitric oxide production of lipopolysaccharide-induced RAW264.7 inflammatory cells.

关 键 词：老鹳草 总鞣质 总黄酮 响应面法 RAW264.7 

分 类 号：R931.6[医药卫生—生药学] R283[医药卫生—药学]