血清miR-141-3p、miR-223-3p水平与新生儿坏死性小肠结肠炎病情严重程度的关系  

Analysis of the relationship between serum miR⁃141⁃3p and miR⁃223⁃3p levels and severity of neonatal necrotizing enterocolitis

作　　者：丁道奎[1] 袁宇航 李延安[1] 杨合英[1] DING Daokui;YUAN Yuhang;LI Yan′an;YANG Heying(Department of Pediatric Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan,China)

机构地区：[1]郑州大学第一附属医院小儿外科,河南郑州450052

出　　处：《实用医学杂志》2025年第7期1050-1055,共6页The Journal of Practical Medicine

基　　金：河南省医学科技攻关计划联合共建项目(编号:LHGJ20210340)。

摘　　要：目的研究分析血清微小核糖核酸-141-3p(miR-141-3p)、miR-223-3p水平与新生儿坏死性小肠结肠炎病情的关系。方法选取2019年1月至2022年1月收治的患有坏死性小肠结肠炎的新生儿80例为研究对象(患病组),根据患儿疾病分期分为Ⅱ期组(n=43)和Ⅲ期组(n=37),选取同期出生的健康新生儿80例为对照组。收集分析一般临床资料,qRT-PCR检测miR-141-3p、miR-223-3p的表达水平;Spearman法分析miR-141-3p、miR-223-3p与病情程度的相关性;多因素logistic回归分析miR-141-3p、miR-223-3p对患儿病情程度的影响因素,绘制受试者工作特征(ROC)曲线分析miR-141-3p、miR-223-3p对患儿病情程度的诊断价值。结果与对照组相比,患病组的miR-141-3p、miR-223-3p相对表达量显著降低(P<0.05);与Ⅱ期组相比,Ⅲ期组的miR-141-3p、miR-223-3p相对表达量显著降低(P<0.05);miR-141-3p、miR-223-3p与病情程度均呈负相关(r=-0.489、-0.496,P<0.05);患儿miR-141-3p、miR-223-3p相对表达量升高为影响患儿病情程度的保护因素(P<0.05);miR-141-3p、miR-223-3p以及两者联合诊断坏死性小肠结肠炎患儿病情程度的AUC分别为0.806、0.783、0.885,联合诊断显著优于miR-141-3p(Z=2.050,P=0.040)、miR-223-3p(Z=2.184,P=0.029)单独诊断。结论在坏死性小肠结肠炎新生儿中,血清miR-141-3p、miR-223-3p相对表达量显著降低,与病情具有密切联系,对病情程度具有一定辅助诊断价值。Objective To investigate and analyze the association between serum microRNA⁃141⁃3p(miR⁃141⁃3p)and miR⁃223⁃3p levels and neonatal necrotizing enterocolitis.Methods Eighty neonates with necrotizing enterocolitis admitted to our hospital between January 2019 and January 2022 were enrolled as subjects.According to the disease stage,they were divided into the stageⅡgroup(n=43)and the stageⅢgroup(n=37).Additionally,80 healthy newborns born during the same period were recruited as the control group.General clinical data were collected and analyzed systematically.The expression levels of miR⁃141⁃3p and miR⁃223⁃3p were detected using quantitative real⁃time PCR(qRT⁃PCR).Spearman′s correlation analysis was performed to evaluate the relationship between miR⁃141⁃3p,miR⁃223⁃3p,and the severity of the disease.Multivariate logistic regression analysis was conducted to assess the impact of miR⁃141⁃3p and miR⁃223⁃3p on the severity of pediatric illness.Furthermore,a Receiver Operating Characteristic(ROC)curve was plotted to determine the diagnostic value of miR⁃141⁃3p and miR⁃223⁃3p in predicting the severity of the patient′s condition.Results Compared with the control group,the relative expression levels of miR⁃141⁃3p and miR⁃223⁃3p in the diseased group were significantly decreased(P<0.05).Similarly,compared with the stageⅡgroup,the relative expression levels of miR⁃141⁃3p and miR⁃223⁃3p in the stageⅢgroup were also markedly reduced(P<0.05).Furthermore,miR⁃141⁃3p and miR⁃223⁃3p exhibited a negative correlation with disease severity(r=-0.489,-0.496,P<0.05).Increased relative expression of miR⁃141⁃3p and miR⁃223⁃3p was identified as a protective factor influencing disease severity in children(P<0.05).The AUC values for diagnosing the severity of necrotizing enterocolitis using miR⁃141⁃3p,miR⁃223⁃3p,and their combination were 0.806,0.783,and 0.885,respectively.Combined diagnosis demonstrated significantly better performance than miR�

关 键 词：微小核糖核酸-141-3p 微小核糖核酸-223-3p 新生儿坏死性小肠结肠炎 病情程度 

分 类 号：R722.1[医药卫生—儿科]