凤梨草莓与森林草莓种间杂种鉴定及果实挥发性成分检测  

Identification of Interspecific Hybrids of Fragaria×ananassa and F.vesca and Detection of Fruit Volatiles

作　　者：成智 李迎梅 隗永青 张宏力 张运涛[1] 王桂霞[1] 钟传飞[1] 常琳琳[1] 高用顺 孙瑞 魏灵芝 李双桃 孙健[1] 董静[1] CHENG Zhi;LI Yingmei;WEI Yongqing;ZHANG Hongli;ZHANG Yuntao;WANG Guixia;ZHONG Chuanfei;CHANG Linlin;GAO Yongshun;SUN Rui;WEI Lingzhi;LI Shuangtao;SUN Jian;DONG Jing(Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences/Beijing Engineering Research Center for Strawberry/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture and Rural Affairs,Beijing 100093,China)

机构地区：[1]北京市农林科学院林业果树研究所/北京市草莓工程技术研究中心/农业部华北地区园艺作物生物学与种质创新重点实验室,北京100093

出　　处：《西北农业学报》2025年第4期709-717,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家特色蔬菜产业技术体系(CARS-24-A-14);北京市农林科学院创新能力建设专项(KJCX20230118、KJCX20240322)。

摘　　要：为筛选果实香气浓的凤梨草莓与森林草莓种间杂种,以栽培品种‘甜查理’为母本、野生株系森林白果为父本进行远缘杂交,获得了种间F1后代,采用形态学标记、流式细胞术、SSR分子标记进行种间杂种鉴定。结果表明:7个种间F1后代的叶片形态特征介于双亲之间,均存在败育,果实为粉红色或红色,其中有5个五倍体和2个六倍体,利用SSR引物Fv000003v1在7个株系中均扩增到父本特异性条带,说明供试种间F1后代均为种间杂种。利用气-质联用仪对种间杂种果实挥发性成分进行检测,结果显示种间杂种的果实挥发性成分数量多于双亲,‘21-509-2’和‘21-509-9’含森林草莓特异性成分,香气更浓,可用于后续香气育种。To screen interspecific hybrids with intense fruit aroma between F.×ananassa and F.vesca,the cultivar‘Sweet charlie’was used as the female parent and crossed with wild accession Senlinbaiguo,7 F1 progeny were obtained.The interspecific hybrid identification was conducted using a combination of morphological markers,flow cytometry,and SSR molecular markers.Fruit volatile compounds in the identified hybrids were qualitatively analyzed using Gas Chromatograph-Mass Spectrometry(GC-MS).The results showed distinct leaf characteristics in the F1 plants,with foliage that was green to dark green,and terminal leaflets that were round to oval,differentiating them from those of Senlinbaiguo.The offspring leaves were significantly thicker than those of F.vesca,and overall,leaf morphology in the F1 progeny were more closely resembled‘Sweet charlie’,the F.×ananassa parent.Seven progeny exhibited sterility to varying degrees.All F1 fruits were pink or red upon ripening,with no white mature fruits observed.Five pentaploid F1 progeny and two hexaploid ones were identified by flow cytometry.The SSR primer Fv000003v1 amplified parent-specific bands in all seven lines.A PCR product about 400 bp was found in Senlinbaiguo and tested offspring plants using gel electrophoresis,producing a PCR product of approximately 400 bp in Senlinbaiguo and the F1 plants,but not in‘Sweet charlie’.This confirmed that the seven F1 progeny were interspecific hybrids.Fruit volatile compounds were detected in the parental lines and four hybrids.The result showed that the number of volatile compounds in the interspecific hybrids was higher than those in the parents.Specifically,the hybrids‘21-509-2’and‘21-509-9’showed increased concentrations of aroma-contributing volatiles,with compounds characteristic of F.vesca,such as ethyl decanoate,ethyl dodecanoate,2-undecanone,and 2-tridecanone,enhancing their aromatic profiles.These two hybrids are suitable for further aroma breeding.

关 键 词：凤梨草莓 森林草莓 种间杂种 果实颜色 染色体倍性 果实挥发性成分 

分 类 号：S668.9[农业科学—果树学]